SERVICE AND APPLICATION FORM
Service procedure explanation:
1.Apply for a Case
2.Commencement of Experiments
3.Conclusion of Experiments
4.End of Case
Please download the appropriate document in“Application Forms”and ask your PRINCIPAL INVESTIGATOR (ADVISER) to sign it.
| Services | Experimental Procedure | Download Application Form | Predicted Experimental Result | Service Staff | Details of Payment | |
|---|---|---|---|---|---|---|
| Provision of Arabidopsis materials |  | Fuhui Wu | Provided free of charge; it is necessary to fill out a Plant Materials Application Form | |||
| Experiments related to transient expression transfection | Plasmid isolation (for protoplasts use) | MACHEREY-NAGEL NucleoBond Xtra plasmid Midiprep kits for plasmid extraction UsingViogene Mini PlusTM Column purification |
| Plasmid DNA concentration 1 μg/μl or more; DNA quality 260/280 = 1.8 or more (nano drop) | Fuhui Wu | 1. NT$300/column 2. Every 12 columns, manpower cost NT$2700. |
| Arabidopsis protoplasts | |  | Fuhui Wu | 1. 1-8 sets, manpower costs, NT$2300, 9-16 sets, manpower costs NT$2650. 2. chemical and consumables NT$500 /8 sets. | ||
| seeding of rice(in vitro) | sowing in 1/2 MS medium | | Fuhui Wu | 1. The applicant is requested to provide the seeds 2. Manpower and chemical costs NT$750/30 seedlings | ||
| Rice protoplasts | | | Fuhui Wu | 1. 1-8 sets, manpower cost NT$2700, 9-16 sets, manpower cost NT$3100。 2.Drugs and consumables NT$700 /8 sets。 | ||
| Construction of CRISPR target vector |  | | send back promoter and target sites sequence data, sequence-confirmed plasmid. | Fuhui Wu | Arabidopsis(less than 3 target sites): 13000元/construction Monocot and dicot except Arabidopsis (less than 4 target sites):16000元/construction Monocot and dicot except Arabidopsis (5-6 target sites):22000元/construction | |