Service method and reference

1. Arabidopsis Protoplasts Transient Expression

Wu FH, Shen SC, Lee LY, Lee SH, Chan MT, Lin CS. 2009 Tape-Arabidopsis sandwich - a simpler Arabidopsis protoplast isolation method. Plant Methods 5:16
https://pubmed.ncbi.nlm.nih.gov/19930690/

2. Rice Protoplasts Transient Expression

Lin CS, Hsu CT, Yang LH, Lee LY, Fu JY, Cheng QW, Wu FH, Hsiao HC, Zhang Y, Zhang R, Chang WJ, Yu CT, Wang W, Liao LJ, Gelvin SB, Shih MC. 2018 Application of protoplast technology to CRISPR/Cas9 mutagenesis: From single cell mutation detection to mutant plant regeneration. Plant Biotech. J. 16(7):1295-1310.
https://pubmed.ncbi.nlm.nih.gov/29230929/

3. Resin Tissue Sectioning

Hsu PK, Tsay YF. 2013 Two Phloem Nitrate Transporters, NRT1.11 and NRT1.12, Are Important for Redistributing Xylem-Borne Nitrate to Enhance Plant Growth. Plant Physiol. 163.
https://pubmed.ncbi.nlm.nih.gov/24006285/

4. Agarose Gel Tissue Sectioning

Wang YY, Tsay YF. 2011 Arabidopsis Nitrate Transporter NRT1.9 Is Important in Phloem Nitrate Transport. Plant Cell 23: 1945-1957.
http://www.plantcell.org/content/23/5/1945

5. Construction of CRISPR Target Vector

a. Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X, Dong Z, Liu YG. 2015 A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants. Mol Plant. 8(8):1274-84.
https://pubmed.ncbi.nlm.nih.gov/25917172/

b. Wang ZP, Xing HL, Dong L, Zhang HY, Han CY, Wang XC, Chen QJ. 2015 Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 21(16)144.
https://pubmed.ncbi.nlm.nih.gov/26193878/