Construction of CRISPR target vector - Experimental Procedure

Rice protoplast transient expression assay

Fill in the information about the experiments in detail, have the application agreed and signed by the project leader

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Applicant provide Gene ID (or gDNA sequence) and CDS sequence

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Core laboratory staff screening target site candidates

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Send back target site candidates file, and applicant can choose 1 to 4 target sites into one vector

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Order oligos for vector construction

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vector construction and single colonies sequence

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send back sequence data and sequence-confirmed plasmid