In planta Particle Bombardment (iPB): A versatile non-culture method for genome editing enabling genotype-independent and DNA-free genome modification in crop species

Ryozo Imai

Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO)

Genome editing has emerged as a promising tool for crop breeding, but its application in elite crop varieties has been limited. Conventional culture-based genome editing methods face challenges in these varieties, necessitating the exploration of genotype-independent editing techniques. Here, we present a novel approach, in planta Particle Bombardment (iPB), for delivering CRISPR/Cas9 ribonucleoproteins into the shoot apical meristems (SAMs). The SAM, with its potential to develop into reproductive tissues, offers a unique opportunity for heritable genome editing events. We employed the iPB method to introduce a semidwarf1 (sd1)-orthologous mutation into wheat, targeting a gene responsible for stem elongation analogous to the rice 'Green Revolution' gene, SD1. By generating a triple sd1 knockout mutant in hexaploid wheat, we achieved a 10% reduction in culm length without compromising yield. As most current wheat varieties carry the Rht1 mutation, which results in shortened culms due to a gain-of-function mutation in the DELLA/GAI gene, the combination of the two GA traits proved valuable for agronomic improvement. We applied the iPB method to barley and introduced a late-heading phenotype in Japanese elite varieties, which are typically early heading, to meet the new demand for grass production. We also extended the iPB method to dicot plants such as soybean, buckwheat, and melon. Furthermore, we developed an iPB-based gene targeting system that offers maximum flexibility for genome modifications in wheat. In summary, our study presents the iPB method as a promising technique for genotype-independent DNA-free genome editing in crops. The successful application of this approach in diverse crop species demonstrates its versatility and potential impact on crop improvement.

講者: Dr. Ryozo Imai (Executive Researcher, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Japan)

主持人: Dr. Tzyy-Jen Chiou

時間:2024/10/21 11:00 AM

地點:農業科技大樓1樓A134演講廳