Study of peptide hormones signaling involved in the plant development
In higher plants, previous studies indicate that the cell-to-cell interaction is mainly mediated by small lipophilic compounds. Until now, increasing number of studies revealed that peptides are also involved in various aspects of plant development including defense responses, callus growth, meristem organization, self-incompatibility, root growth, leaf shape regulation, nodule development and organ abscission etc. Completion of the Arabidopsis genome has reveal that this plant has ten times more predicted peptide transporter than other sequenced organisms. Compared with the approximate 58 receptorlike kinases (RLKs) known in human, there are over 400 potential RLKs in plants. In Arabidopsis genome contains > 210 lucine rich repeat receptor-like kinases (LRR-RLKs) genes. The LRR domain is known to be involved in protein-protein or protein-peptide interactions. Therefore, it is expected that the plants may have a much greater number of signaling peptides playing prominent roles in the plant development. Currently, only a limited number the biologically active peptides their mature sequences and perceptive receptor are determined and verified. The peptide hormone can interact with LRR-RLKs on the cytoplasm membrane and produce downstream signal transduction. We have integrated and developed several comprehensive research strategies for the discovery of novel peptide ligands, corresponding signaling pathways and perceptive receptors involved in the plant development. In combination with the comprehensive proteomic study of peptide signaling, the outcome is expected to sketch a more detail view of peptide hormone function in plants, and will clarify the differences and similarities in peptide signal transduction between plants and animal. Ultimately, revealing the interplay of the signaling network will help us to manipulate the pathogenesis defense, stress tolerance and other development functions in plants.
Development of high-throughput mass spectrometry based OMICS technologies
The discovery of the important biomarkers and signaling molecules can be used to study their function, develop early diagnostic method and further develop potential therapeutic agents. Recent developments on the mass spectrometry had greatly spanned the possibility of full proteome or metabolome characterization. Based on the mass spectrometry, we are interesting on the development of new proteomics and metabolomics techniques for the discovery of important cellular signaling events. We expect our efforts will eventually establish a general and versatile systems biology research platform and can be finally applied to the development of potential therapeutic drugs and improvement of crop productivity.
Associate Research Fellow
2015- Tenured Associate Research Fellow, Agricultural Biotechnology Research Center, Academia Sinica.
2015- Associate Professor, Department of Chemistry, National Chiayi University.
2015- Associate Professor, Institute of Biotechnology, National Taiwan University.
2015- Associate Professor, Biotechnology Center, National Chung Hsing University.
2015- Associate Professor, Institute of Bioscience and Biotechnology, National Taiwan Ocean University.
2009- Executive PI of Metabolomics Core Facility, Scientific Instrument Service Center, Academia Sinica
2010-2015 Assistant Professor, Institute of Biotechnology, National Taiwan University.
2009-2015 Assistant Professor, Biotechnology Center, National Chung Hsing University.
2007-2015 Assistant Professor, Dep. of Bioscience and Biotech., National Taiwan Ocean University.
2007-2015 Assistant Research Fellow, Agricultural Biotechnology Research Center, Academia Sinica.
2002-2006 Postdoctoral Research Fellow, Institute of Chemistry, Academia Sinica.
2003 Visiting Scholar, Institute for Systems Biology, Seattle, WA, USA
2016 Junior Research Investigators Award, Academia Sinica.
2016 Career Development Award, Academia Sinica.
2015 Professor CY Lin Memorial Award for Innovative Research Program
2015 The Shang-Fa Yang Young Scientist Award, The Shang-Fa Yang Memorial Foundation.
2013 TSMS Young Scholar Award, Taiwan Society for Mass Spectrometry.
2008 Biotechnology Creativity Award, Industrial Development Bureau, Ministry of Economic Affairs.
2015- Council Member, Asia Oceania Agricultural Proteomics Organization.
2015- Executive Council Member, Taiwan Society for Mass Spectrometry
2012-2015 Chair of Finance Committee/Academic Committee, Taiwan Society for Mass Spectrometry
2015- Elected Council Member, Taiwan Society for Mass Spectrometry
2014- Elected Council Member, Taiwan Society for Proteomics
2012-2015 Chair of Finance Committee/Training Course Committee, Taiwan Society for Mass Spectrometry
2012-2015 Elected Council Member, Taiwan Society for Mass Spectrometry
2011-2014 Elected Council Member, Taiwan Society for Proteomics
2009-2012 Elected Council Member/Training Course Committee, Taiwan Society for Mass Spectrometry
2006-2009 Elected Council Member, Taiwan Society for Mass Spectrometry