A salt-regulated peptide derived from the CAP superfamily protein negatively regulates Salt-stress tolerance in Arabidopsis
High salinity has negative impacts on plant growth through altered water uptake and ion-specific toxicities. Plants have therefore evolved an intricate regulatory network in which plant hormones play significant roles in modulating physiological responses to salinity. However, current understanding of the plant peptides involved in this regulatory network remains limited. Here, we identified a salt-regulated peptide in Arabidopsis. The peptide was 11 aa and was derived from the C terminus of a cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 proteins (CAP) superfamily. This peptide was found by searching homologues in Arabidopsis using the precursor of a tomato CAP-derived peptide (CAPE) that was initially identified as an immune signal. In searching for a CAPE involved in salt responses, we screened CAPE precursor genes that showed salt-responsive expression and found that the PROAtCAPE1 (AT4G33730) gene was regulated by salinity. We confirmed the endogenous Arabidopsis CAP-derived peptide 1 (AtCAPE1) by mass spectrometry and found that a key amino acid residue in PROAtCAPE1 is critical for AtCAPE1 production. Moreover, although PROAtCAPE1 was expressed mainly in the roots, AtCAPE1 was discovered to be upregulated systemically upon salt treatment. The salt-induced AtCAPE1 negatively regulated salt tolerance by suppressing several salt-tolerance genes functioning in the production of osmolytes, detoxification, stomatal closure control, and cell membrane protection. This discovery demonstrates that AtCAPE1, a homologue of tomato immune regulator CAPE1, plays an important role in the regulation of salt stress responses. Our discovery thus suggests that the peptide may function in a trade-off between pathogen defence and salt tolerance.同研究人員:Pei-Shan Chien, Hong Gil Nam and Yet-Ran Chen
Yet-Ran Chen
Research Fellow
CURRENT POSITION
2024- Research Fellow, Agricultural Biotechnology Research Center, Academia Sinica.
2024- President, Taiwan Society for Mass Spectrometry.
2015- Professor, Biotechnology Center, National Chung Hsing University.
2009- Executive PI of Metabolomics Core Facility, Academia Sinica
PROFESSIONAL EXPERIENCES
2015-2024 Tenured Associate Research Fellow, Agricultural Biotechnology Research Center, Academia Sinica.
2007-2015 Assistant Research Fellow, Agricultural Biotechnology Research Center, Academia Sinica.
2006-2007 Assistant Professor, Dep. of Bioscience and Biotech., National Taiwan Ocean University.
2002-2006 Postdoctoral Research Fellow, Institute of Chemistry, Academia Sinica.
2003 Visiting Scholar, Institute for Systems Biology, Seattle, WA, USA
AWARDS
2025 Outstanding Scholar Award, Foundation for the Advancement of Outstanding Scholarship (FAOS)
2024 Investigation Research Grant/Award, Academia Sinica
2024 Distinguished Scholar Award, Taiwan Society for Mass Spectrometry
2016 Junior Research Investigators Award, National Award by Academia Sinica.
2016 Career Development Award, Academia Sinica.
2015 Professor CY Lin Memorial Award for Innovative Research Program, The Chu-Yung Lin Memorial Foundation and Taiwan Society for Plant Biologists (TSPB).
2015 The Shang-Fa Yang Young Scientist Award, The Shang-Fa Yang Memorial Foundation.
2013 TSMS Young Scholar Award, Taiwan Society for Mass Spectrometry.
2008 Biotechnology Creativity Award, Industrial Development Bureau, Ministry of Economic Affairs.
HONORS
2024- President, Taiwan Society for Mass Spectrometry.
2024 Significant Discovery, Academia Sinica 2023 Yearly Report
2021- Council Member, Taiwan Metabolomics Society.
2016 Significant Discovery, Academia Sinica 2015 Yearly Report
2015 Significant Discovery, Academia Sinica 2014 Yearly Report
2015- Council Member, Asia Oceania Agricultural Proteomics Organization.
2011- Elected Council Member / Executive Council Member (2021-), Taiwan Society for Proteomics.
2006- Elected Council Member / Chair of Publication Committee (2021-) / Executive Council Member (2015-) / Chair of Finance Committee (2015-21) / Academic Committee (2015-) / Training Course Committee (2009-12), Taiwan Society for Mass Spectrometry.