Quantitative Peptidomics Study Reveals a Wound-Induced Peptide from PR-1 Regulates Immune Signaling in Tomato
Many important cell-to-cell communication events in multicellular organisms are mediated by peptides, but only a few peptides have been identified in plants. In an attempt to address the difficulties in identifying plant signaling peptides, we developed a novel peptidomics approach and used this approach to discover defense signaling peptides in plants. In addition to the canonical peptide systemin, several novel peptides were confidently identified in tomato (Solanum lycopersicum) and quantified to be induced by both wounding and methyl jasmonate (MeJA). A wounding or wounding plus MeJA-induced peptide derived from the pathogenesis-related protein 1 (PR-1) family was found to induce significant antipathogen and minor antiherbivore responses in tomato. This study highlights a role for PR-1 in immune signaling and suggests the potential application of plant endogenous peptides in efforts to defeat biological threats in crop production. As PR-1 is highly conserved across many organisms and the putative peptide from At-PR1 was also found to be bioactive in Arabidopsis thaliana, our results suggest that this peptide may be useful for enhancing resistance to stress in other plant species.同研究人員:Ying-Lan Chen, Chi-Ying Lee, Kai-Tan Cheng, Wei-Hung Chang, Rong-Nan Huang, Hong Gil Nam and Yet-Ran Chen
Yet-Ran Chen
Associate Research Fellow
PROFESSIONAL EXPERIENCES
2015- Tenured Associate Research Fellow, Agricultural Biotechnology Research Center, Academia Sinica.
2015- Associate Professor, Department of Chemistry, National Chiayi University.
2015- Associate Professor, Institute of Biotechnology, National Taiwan University.
2015- Associate Professor, Biotechnology Center, National Chung Hsing University.
2015- Associate Professor, Institute of Bioscience and Biotechnology, National Taiwan Ocean University.
2009- Executive PI of Metabolomics Core Facility, Scientific Instrument Service Center, Academia Sinica
2010-2015 Assistant Professor, Institute of Biotechnology, National Taiwan University.
2009-2015 Assistant Professor, Biotechnology Center, National Chung Hsing University.
2007-2015 Assistant Professor, Dep. of Bioscience and Biotech., National Taiwan Ocean University.
2007-2015 Assistant Research Fellow, Agricultural Biotechnology Research Center, Academia Sinica.
2002-2006 Postdoctoral Research Fellow, Institute of Chemistry, Academia Sinica.
2003 Visiting Scholar, Institute for Systems Biology, Seattle, WA, USA
AWARDS
2016 Junior Research Investigators Award, Academia Sinica.
2016 Career Development Award, Academia Sinica.
2015 Professor CY Lin Memorial Award for Innovative Research Program
2015 The Shang-Fa Yang Young Scientist Award, The Shang-Fa Yang Memorial Foundation.
2013 TSMS Young Scholar Award, Taiwan Society for Mass Spectrometry.
2008 Biotechnology Creativity Award, Industrial Development Bureau, Ministry of Economic Affairs.
HONORS
2015- Council Member, Asia Oceania Agricultural Proteomics Organization.
2015- Executive Council Member, Taiwan Society for Mass Spectrometry
2012-2015 Chair of Finance Committee/Academic Committee, Taiwan Society for Mass Spectrometry
2015- Elected Council Member, Taiwan Society for Mass Spectrometry
2014- Elected Council Member, Taiwan Society for Proteomics
2012-2015 Chair of Finance Committee/Training Course Committee, Taiwan Society for Mass Spectrometry
2012-2015 Elected Council Member, Taiwan Society for Mass Spectrometry
2011-2014 Elected Council Member, Taiwan Society for Proteomics
2009-2012 Elected Council Member/Training Course Committee, Taiwan Society for Mass Spectrometry
2006-2009 Elected Council Member, Taiwan Society for Mass Spectrometry