Tunnel Frit: A Nonmetallic In-Capillary Frit for Nanoultra High-Performance Liquid Chromatography - Mass Spectrometry Applications
n this study, an easy method to fabricate a durable in-capillary frit was developed for use in nanoflow liquid chromatography (nanoLC). A small orifice was tunneled into the sol_gel frit during the polymerization process resulting in the simple fabrication of a tunnel frit. A short packing tunnel frit column (2 cm, C18 particles) was able to sustain over 10 000 psi continuous liquid flow for 10 days without observation of particle loss, and back pressure variation was less than 5%. The tunnel frit was successfully applied to the fabrication of nanoflow ultra high-performance liquid chromatography (nano-UHPLC) trap and analytical columns. In the analysis of tryptic peptides, the tunnel frit trap and analytical columns were demonstrated to have high separation efficiency and sensitivity. In analysis of phosphopeptides, the use of the nonmetallic tunnel frit column showed better sensitivity than the metallic frit column. This design can facilitate the preparation of nano-HPLC and nano-UHPLC columns and the packing material can easily be refilled when the column is severely contaminated or clogged.
Yet-Ran Chen
Associate Research Fellow
PROFESSIONAL EXPERIENCES
2015- Tenured Associate Research Fellow, Agricultural Biotechnology Research Center, Academia Sinica.
2015- Associate Professor, Department of Chemistry, National Chiayi University.
2015- Associate Professor, Institute of Biotechnology, National Taiwan University.
2015- Associate Professor, Biotechnology Center, National Chung Hsing University.
2015- Associate Professor, Institute of Bioscience and Biotechnology, National Taiwan Ocean University.
2009- Executive PI of Metabolomics Core Facility, Scientific Instrument Service Center, Academia Sinica
2010-2015 Assistant Professor, Institute of Biotechnology, National Taiwan University.
2009-2015 Assistant Professor, Biotechnology Center, National Chung Hsing University.
2007-2015 Assistant Professor, Dep. of Bioscience and Biotech., National Taiwan Ocean University.
2007-2015 Assistant Research Fellow, Agricultural Biotechnology Research Center, Academia Sinica.
2002-2006 Postdoctoral Research Fellow, Institute of Chemistry, Academia Sinica.
2003 Visiting Scholar, Institute for Systems Biology, Seattle, WA, USA
AWARDS
2016 Junior Research Investigators Award, Academia Sinica.
2016 Career Development Award, Academia Sinica.
2015 Professor CY Lin Memorial Award for Innovative Research Program
2015 The Shang-Fa Yang Young Scientist Award, The Shang-Fa Yang Memorial Foundation.
2013 TSMS Young Scholar Award, Taiwan Society for Mass Spectrometry.
2008 Biotechnology Creativity Award, Industrial Development Bureau, Ministry of Economic Affairs.
HONORS
2015- Council Member, Asia Oceania Agricultural Proteomics Organization.
2015- Executive Council Member, Taiwan Society for Mass Spectrometry
2012-2015 Chair of Finance Committee/Academic Committee, Taiwan Society for Mass Spectrometry
2015- Elected Council Member, Taiwan Society for Mass Spectrometry
2014- Elected Council Member, Taiwan Society for Proteomics
2012-2015 Chair of Finance Committee/Training Course Committee, Taiwan Society for Mass Spectrometry
2012-2015 Elected Council Member, Taiwan Society for Mass Spectrometry
2011-2014 Elected Council Member, Taiwan Society for Proteomics
2009-2012 Elected Council Member/Training Course Committee, Taiwan Society for Mass Spectrometry
2006-2009 Elected Council Member, Taiwan Society for Mass Spectrometry