Tunnel Frit: A Nonmetallic In-Capillary Frit for Nanoultra High-Performance Liquid Chromatography - Mass Spectrometry Applications
n this study, an easy method to fabricate a durable in-capillary frit was developed for use in nanoflow liquid chromatography (nanoLC). A small orifice was tunneled into the sol_gel frit during the polymerization process resulting in the simple fabrication of a tunnel frit. A short packing tunnel frit column (2 cm, C18 particles) was able to sustain over 10 000 psi continuous liquid flow for 10 days without observation of particle loss, and back pressure variation was less than 5%. The tunnel frit was successfully applied to the fabrication of nanoflow ultra high-performance liquid chromatography (nano-UHPLC) trap and analytical columns. In the analysis of tryptic peptides, the tunnel frit trap and analytical columns were demonstrated to have high separation efficiency and sensitivity. In analysis of phosphopeptides, the use of the nonmetallic tunnel frit column showed better sensitivity than the metallic frit column. This design can facilitate the preparation of nano-HPLC and nano-UHPLC columns and the packing material can easily be refilled when the column is severely contaminated or clogged.
Yet-Ran Chen
Research Fellow
CURRENT POSITION
2024- Research Fellow, Agricultural Biotechnology Research Center, Academia Sinica.
2024- President, Taiwan Society for Mass Spectrometry.
2015- Professor, Biotechnology Center, National Chung Hsing University.
2009- Executive PI of Metabolomics Core Facility, Academia Sinica
PROFESSIONAL EXPERIENCES
2015-2024 Tenured Associate Research Fellow, Agricultural Biotechnology Research Center, Academia Sinica.
2007-2015 Assistant Research Fellow, Agricultural Biotechnology Research Center, Academia Sinica.
2006-2007 Assistant Professor, Dep. of Bioscience and Biotech., National Taiwan Ocean University.
2002-2006 Postdoctoral Research Fellow, Institute of Chemistry, Academia Sinica.
2003 Visiting Scholar, Institute for Systems Biology, Seattle, WA, USA
AWARDS
2025 Outstanding Scholar Award, Foundation for the Advancement of Outstanding Scholarship (FAOS)
2024 Investigation Research Grant/Award, Academia Sinica
2024 Distinguished Scholar Award, Taiwan Society for Mass Spectrometry
2016 Junior Research Investigators Award, National Award by Academia Sinica.
2016 Career Development Award, Academia Sinica.
2015 Professor CY Lin Memorial Award for Innovative Research Program, The Chu-Yung Lin Memorial Foundation and Taiwan Society for Plant Biologists (TSPB).
2015 The Shang-Fa Yang Young Scientist Award, The Shang-Fa Yang Memorial Foundation.
2013 TSMS Young Scholar Award, Taiwan Society for Mass Spectrometry.
2008 Biotechnology Creativity Award, Industrial Development Bureau, Ministry of Economic Affairs.
HONORS
2024- President, Taiwan Society for Mass Spectrometry.
2024 Significant Discovery, Academia Sinica 2023 Yearly Report
2021- Council Member, Taiwan Metabolomics Society.
2016 Significant Discovery, Academia Sinica 2015 Yearly Report
2015 Significant Discovery, Academia Sinica 2014 Yearly Report
2015- Council Member, Asia Oceania Agricultural Proteomics Organization.
2011- Elected Council Member / Executive Council Member (2021-), Taiwan Society for Proteomics.
2006- Elected Council Member / Chair of Publication Committee (2021-) / Executive Council Member (2015-) / Chair of Finance Committee (2015-21) / Academic Committee (2015-) / Training Course Committee (2009-12), Taiwan Society for Mass Spectrometry.