Publications
| Lin, Choun-Sea*; Hsu, Chen-Tran; Chiu, Chi-Chou; Hsiao, Pao-Yuan; Chih-Yu, Lin; (Cheng, Sychyi); Lin, Yao-Cheng; Yang, Yu-Liang; Wu, Fu-Huei; Harn, Horng-Jyh; Lin, Shinn-Zong (2024) Transgene-Free CRISPR/Cas9-Mediated Gene Editing through Protoplast-to-Plant Regeneration Enhances Active Compounds in Salvia miltiorrhiza. PLANT BIOTECHNOLOGY JOURNAL, 22 (6), 1549-1551 |
| Hsu CT, Chiu CC, Hsiao PY, Lin CY, Cheng S, Lin YC, Yang YL, Wu FH, Harn HJ, Lin SZ, (Lin CS)* (2024) Transgene-free CRISPR/Cas9-mediated gene editing through protoplast-to-plant regeneration enhances active compounds in Salvia miltiorrhiza.. Plant biotechnology journal, pbi.14285, pbi.14285 |
| Wu FH, Hsu CT, (Lin CS*) (2023) Targeted Insertion in Nicotiana benthamiana Genomes via Protoplast Regeneration.. Methods in Molecular Biology, 2653, 297-315 |
| Lin CC, Lee WJ, Zeng CY, Chou MY, Lin TJ, (Lin CS), Ho MC, Shih MC (2023) SUB1A-1 anchors a regulatory cascade for epigenetic and transcriptional controls of submergence tolerance in rice.. PNAS nexus, 2(7), pgad229 |
| Wu FH, Yuan YH, Hsu CT, Cheng QW, Lin CS (2022) Application of Protoplast Regeneration to CRISPR/Cas9 Mutagenesis in Nicotiana tabacum.. Methods in molecular biology (Clifton, N.J.), 2464, 49-64 |
| Fu-Hui Wu, Yu-Hsuan Yuan, Chen-Tran Hsu, Qiao-Wei Cheng, Choun-Sea Lin (2022) Application of Protoplast Regeneration to CRISPR/Cas9 Mutagenesis in Nicotiana tabacum. Methods in Molecular Biology, vol. 2464 |
| Choun-Sea Lin, Chen-Tran Hsu, Yu-Hsuan Yuan, Po-Xing Zheng, Fu-Hui Wu, Qiao-Wei Cheng, Yu-Lin Wu, Ting-Li Wu, Steven Lin, Jin-Jun Yue, Ying-Huey Cheng, Shu-I Lin, Ming-Che Shih, Jen Sheen, Yao-Cheng Lin (2022) DNA-free CRISPR-Cas9 gene editing of wild tetraploid tomato Solanum peruvianum using protoplast regeneration. Plant Physiology, 188(4): 1917–1930 DOI | News and Views | Cover Image | First Author Profile |
| Fan-Chen Huang, Shin-Fei Chi, Pei-Ru Chien, Yin-Tzu Liu, Hsin-Nung Chang, Choun-Sea Lin , Hau-Hsuan Hwang＊ (2021) Arabidopsis RAB8A, RAB8B and RAB8D Proteins Interact with Several RTNLB Proteins and are Involved in the Agrobacterium tumefaciens Infection Process. Plant & Cell Physiology, 62(10), 1572-1588 |
| Yue Jin-Jun, Yuan Jin-Ling, Wu Fu-Hui, Yuan Yu-Hsuan, Cheng Qiao-Wei, Hsu Chen-Tran, Lin Choun-Sea* (2021) Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application. Frontiers in Genome Editing, 3, 1 |
| Hsu Chen-Tran, Lee Wen-Chi, Cheng Yu-Jung, Yuan Yu-Hsuan, Wu Fu-Hui, Lin Choun-Sea* (2021) Genome Editing and Protoplast Regeneration to Study Plant–Pathogen Interactions in the Model Plant Nicotiana benthamiana. Frontiers in Genome Editing, 2, 1 |
| Hsu Chen-Tran, Yuan Yu-Hsuan, Lin Yao-Cheng, Lin Steven, Cheng Qiao-Wei, Wu Fu-Hui, Sheen Jen, Shih Ming-Che, Lin Choun-Sea (2021) Efficient and Economical Targeted Insertion in Plant Genomes via Protoplast Regeneration. The CRISPR Journal, |
| Tong CG, Wu FH, Yuan YH, Chen YR, Lin CS* (2020) High‐efficiency CRISPR/Cas‐based editing of Phalaenopsis orchid MADS genes. Plant Biotechnology Journal, 18(4) 889-891 |
| Yang DM, Fu TF, Lin CS, Chiu TY, Huang CC, Huang HY, Chung MW, Lin YS, Manurung RV Nhi NPN, Chang YF (2020) High-performance FRET biosensors for single-cell and in vivo lead detection. Biosensors and Bioelectronics, 168, 112571 |
| Yu Y, Yu PC, Chang WJ, Yu K, Lin CS* (2020) Plastid Transformation: How Does it Work? Can it Be Applied to Crops? What Can it Offer? International Journal of Molecular Sciences, 21(14) |
| Yue JJ, Hong CY, Wei PC, Tsai YC, Lin CS* (2020) How to start your monocot CRISPR/Cas project: plasmid design, efficiency detection, and offspring analysis. RICE, 13(1) |
| Guo ZH, Ma PF, Yang GQ, Hu JY, Liu YL, Xia EH, Zhong MC, Zhao L, Sun GL, Xu YX, Zhao YJ, Zhang YC, Zhang YX, Zhang XM, Zhou MY, Guo Y, Guo C, Liu JX, Ye XY, Chen YM, Yang Y, Han B, Lin CS*, Lu Y*, Li DZ* (2019) Genome sequences provide insights into the reticulate origin and unique traits of woody bamboos. Molecular plant, 12(10),1353-1365 |
| Hsu CT, Cheng YJ, Yuan YH, Hung WF, Cheng QW, Wu FH, Lee LY, Gelvin SB, Lin CS* (2019) Application of Cas12a and nCas9-activation-induced cytidine deaminase for genome editing and as a non-sexual strategy to generate homozygous/multiplex edited plants in the allotetraploid genome of tobacco. Plant Molecular Biology, 101(4-5),355-371 |
| Jiang JZ, Kuo CH, Chen BH, Chen MK, Lin CS, Ho SL (2018) Effects of OsCDPK1 on the Structure and Physicochemical Properties of Starch in Developing Rice Seeds. International Journal of Molecular Sciences, 19(10), 3247 |
| Lin CS*, Hsu CT, Yang LH, Lee LY, Fu JY, Cheng QW, Wu FH, Hsiao HC, Zhang Y, Zhang R, Chang WJ, Yu CT, Wang W, Liao LJ, Gelvin SB, Shih MC (2018) Application of protoplast technology to CRISPR/Cas9 mutagenesis: From single cell mutation detection to mutant plant regeneration. Plant Biotechnology Journal, 16, 1295-1310 |
| Yeh Hsuan-Yu, Lin Choun-Sea, de Jong Hans, Chang Song-Bin (2017) Two reported cytotypes of the emergent orchid model species Erycina pusilla are two different species. Euphytica, 213(10), 233-233 |
| Yuan JL, Yue JJ, Gu XP, Lin CS* (2017) Flowering of Woody Bamboo in Tissue Culture Systems. Frontiers in Plant Science, 8, 1589 |
| Lin CS*, Chen JJ, Chiu CC, Hsiao HC, Yang CJ, Jin XH, Leebens-Mack J, dePamphilis CW, Huang YT, Yang LH, Chang WJ, Kui L, Wong GK, Hu JM, Wang W, Shih MC* (2017) Concomitant loss of NDH complex-related genes within chloroplast and nuclear genomes in some orchids. The Plant journal : for cell and molecular biology, 90(5), 994-1006 |
| Lin CS*, Hsu CT, Liao C, Chang WJ, Chou ML, Huang YT, Chen JJ, Ko SS, Chan MT, Shih MC (2016) Transcriptome-wide analysis of the MADS-box gene family in the orchid Erycina pusilla. Plant Biotechnology Journal, 14(1), 284-298 |
| Yan-Lei Feng, Susann Wicke, Jian-Wu Li, Yu Han, Choun-Sea Lin, De-Zhu Li, Ting-Ting Zhou, Wei-Chang Huang, Lu-Qi Huang and Xiao-Hua Jin (2016) Lineage-specific reductions of plastid genomes in an orchid tribe with partially and fully mycoheterotrophic species. Genome Biology and Evolution, 8 (7), 2164-2175 |
| Daniell H, Lin CS, Yu M, Chang WJ (2016) Chloroplast genomes: diversity, evolution, and applications in genetic engineering. Genome Biology, 17(1), 134 |
| Ting CK, Lin CS, Chan MT, Chen JW, Chuang SY, Huang YT (2016) A Genetic Algorithm for Diploid Genome Reconstruction Using Paired-End Sequencing. PloS one, 11(11), e0166721 |
| Yi-Lin Hsieh & Ching-Fang Lu & Bing-Yu Chiang & Shu-Chien Liao &Rita P.-Y. Chen & Choun-Sea Lin & Ai-Yu Wang & Chien-Chih Yang (2016) Molecular Characterization of Ethylene Response Sensor 1 (BoERS1) in Bambusa oldhamii . PLANT MOLECULAR BIOLOGY REPORTER, 34(2), 387-398 |
| Sean Vincent Burke, Choun-Sea Lin, William Patrick Wysocki, Lynn G Clark, Melvin R Duvall (2016) Phylogenomics and plastome evolution of tropical forest grasses (Leptaspis, Streptochaeta: Poaceae). Frontiers in Plant Science, 7, 1993 |
| Lin CS*, Chen JJ, Huang YT, Chan MT, Daniell H, Chang WJ, Hsu CT, Liao DC, Wu FH, Lin SY, Liao CF, Deyholos MK, Wong GK, Albert VA, Chou ML, Chen CY, Shih MC (2015) The location and translocation of ndh genes of chloroplast origin in the Orchidaceae family. Scientific Reports, 5, 9040 |
| Tracey A Ruhlman, Wan-Jung Chang, Jeremy JW Chen, Yao-Ting Huang, Ming-Tsair Chan, Jin Zhang, De-Chih Liao, John C Blazier, Xiaohua Jin, Ming-Che Shih, Robert K Jansen and Choun-Sea Lin* (2015) NDH expression marks major transitions in plant evolution and reveals coordinate intracellular gene loss. BMC Plant Biology, 15, 100 |
| Hsuan-Yu Yeh, Choun-Sea Lin, Song-Bin Chang (2015) Cytogenetic and cytometric analyses in artificial intercytotypic hybrids of the emergent orchid model species Erycina pusilla. Euphytica, 206(2), 533-539 |
| Lee SH, Li CW, Koh KW, Chuang HY, Chen YR, Lin CS, Chan MT (2014) MSRB7 reverses oxidation of GSTF2/3 to confer tolerance of Arabidopsis thaliana to oxidative stress. Journal of Experimental Botany, 65(17), 5049-5062 |
| Shih MC, Chou ML, Yue JJ, Hsu CT, Chang WJ, Ko SS, Liao DC, Huang YT, Chen JJ, Yuan JL, Gu XP, Lin CS* (2014) BeMADS1 is a key to delivery MADSs into nucleus in reproductive tissues-De novo characterization of Bambusa edulis transcriptome and study of MADS genes in bamboo floral development. BMC Plant Biology, 14, 179 |
| Lee SH, Li CW, Liau CH, Chang PY, Liao LJ, Lin CS, Chan MT (2014) Establishment of an Agrobacterium-mediated genetic transformation procedure for the experimental model orchid Erycina pusilla. PLANT CELL TISSUE AND ORGAN CULTURE, 120(1), 211-220 |
| Lin CS*, Chen JJ, Huang YT, Hsu CT, Lu HC, Chou ML, Chen LC, Ou CI, Liao DC, Yeh YY, Chang SB, Shen SC, Wu FH, Shih MC, Chan MT (2013) Catalog of Erycina pusilla miRNA and categorization of reproductive phase-related miRNAs and their target gene families. Plant Molecular Biology, 82(1-2), 193-204 |
| Liao SC, Lin CS, Wang AY*, Sung HY* (2013) Differential expression of genes encoding acid invertases in multiple shoots of bamboo in response to various phytohormones and environmental factors. Journal of Agricultural and Food Chemistry, 61(18), 4396-4405 |
| Chou ML, Shih MC, Chan MT, Liao SY, Hsu CT, Haung YT, Chen JJ, Liao DC, Wu FH, Lin CS* (2013) Global transcriptome analysis and identification of a CONSTANS-like gene family in the orchid Erycina pusilla. Planta, 237(6), 1425-1441 |
| Pan IC, Liao DC, Wu FH, Daniell H, Singh ND, Chang C, Shih MC, Chan MT*, Lin CS* (2012) Complete Chloroplast Genome Sequence of an Orchid Model Plant Candidate: Erycina pusilla Apply in Tropical Oncidium Breeding. PloS one, 7(4), e34738 |
| Lee LY, Wu FH, Hsu CT, Shen SC, Yeh HY, Liao DC, Fang MJ, Liu NT, Yen YC, Dokladal L, Sykorova E, Gelvin SB, Lin CS* (2012) Screening a cDNA Library for Protein-Protein Interactions Directly in Planta. The Plant cell, 24(5), 1746-1759 |
| Li CW, Lee SH, Chieh PS, Lin CS, Wang YC, Chan MT (2012) Arabidopsis Root-Abundant Cytosolic Methionine Sulfoxide Reductase B Genes MsrB7 and MsrB8 are Involved in Tolerance to Oxidative Stress. Plant & Cell Physiology, 53(10), 1707-1719 |
| Hsu CT, Liao DC, Wu FH, Liu NT, Shen SC, Chou SJ, Tung SY, Yang CH, Chan MT*, Lin CS* (2011) Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of Oncidium Gower Ramsey. BMC Plant Biology, 11, 60 |
| Chiu YT, Lin CS, Chang C (2011) In vitro fruiting and seed production in Erycina pusilla. Propagation of Ornamental Plants, 11(3), 131-136 |
| Yeh SH, Lin CS, Wu FH, Wang AY (2011) Analysis of the expression of BohLOL1, which encodes an LSD1-like zinc finger protein in Bambusa oldhamii. Planta, 234(6), 1179-1189 |
| Wu FH, Chan MT, Liao DC, Hsu CT, Lee YW, Daniell H, Duvall MR, Lin CS* (2010) Complete chloroplast genome of Oncidium Gower Ramsey and evaluation of molecular markers for identification and breeding in Oncidiinae. BMC Plant Biology, 10, 68 |
| Chen CY, Hsieh MH, Yang CC, Lin CS, Wang AY (2010) Analysis of the cellulose synthase genes associated with primary cell wall synthesis in Bambusa oldhamii. Phytochemistry, 71(11-12), 1270-1279 |
| Pan IC, Li CW, Su RC, Cheng CP, Lin CS, Chan MT* (2010) Ectopic expression of an EAR motif deletion mutant of SlERF3 enhances tolerance to salt stress and Ralstonia solanacearum in tomato. Planta, 232(5), 1075-1086 |
| Lan TH, Hong PI, Huang, CC, Chang WC, and Lin CS* (2009) Developmentof a system for high-frequency direct somatic embryogenesis from leaf tissues of Drimiopsis kirkii Baker (Giant Squill) . IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 45(1), 44-47 |
| Wu FH, Kan DP, Lee SB, Daniell H, Lee YW, Lin CC, Lin NS, Lin CS* (2009) Complete nucleotide sequence of Dendrocalamus latiflorus and Bambusa oldhamii chloroplast genomes. Tree physiology, 29(6), 847-856 |
| Wu FH, Liu NT, Chou SJ, Shen SC, Chang B CH, Pan CY, Lin CS* (2009) Identification of gene transcript accumulation repressed in three albino mutants of Bambusa edulis Munro. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 89, 2308-2316 |
| Fan SC, Lin CS, PK Hsu, Lin SH Tsay YF (2009) Arabidopsis nitrate transporter NRT1.7, expressed in phloem, is responsible for source-to-sink remobilization of nitrate. Plant Cell, 21, 2750-2761 |
| Wu FH, Shen SC, Lee LY, Lee SH, Chan MT, Lin CS* (2009) Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method. Plant Methods, 5(1), 16 |
| Lee CY Ser HF, Chen HW, Lin CS, Chu TM, Yang PC, Tsay HS, Chen JJW* (2008) The anticancer effects of tanshinone I in human non-small-cell lung cancer. MOLECULAR CANCER THERAPEUTICS, 7(11), 3527-3538 |
| Lin CS, Liu NT, Liao DC, Yu JS, Tsao CH, Lin CH, Sun CW, Jane WN, Tsay HS, Chen JJW, Lai EM, Lin NS, Chang WC, Lin CC* (2008) Differential protein expression of two photosystem II subunits, PsbO and PsbP in an albino mutant of Bambusa edulis Murno with chloroplast DNA aberration . JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 133, 270-277 |
| Lin SH, Kuo HF, Canivenc G, Lin CS, Lepetit M, Hsu PK, Tillard P, Lin HL, Wang YY, Tsai CB, Gojon A, Tsay YF* (2008) Mutation of the AtNRT1.5 nitrate transporter gene causes defective root-to-shoot nitrate transport. Plant Cell, 20(9), 2514-2528 |
| Liu NT, Wu FH, Tsay HS, Chang WC, and Lin CS* (2008) Establishment of a cDNA library from Bambusa edulis in vitro-grown shoot. PLANT CELL TISSUE AND ORGAN CULTURE, 95(1), 21-27 |
| Liu NT, Jane WN, Tsay HS, Wu H, Chang WC, Lin CS* (2007) Chloroplast genome aberration in micropropagation-derived albino Bambusa edulis mutants, ab1 and ab2. PLANT CELL TISSUE AND ORGAN CULTURE, 88,147-156 |
| Lin CS*, Kalpana K, Chang WC, Lin NS (2007) Improving multiple shoot proliferation in bamboo mosaic virus-free Bambusa oldhamii Munro propagation by liquid culture. HORTSCIENCE, 42(5), 1243-1246 |
| Lin CS*, Liang CJ, Hsaio HW, Lin, MJ, Chang WC* (2007) In vitro flowering of green and albino Dendrocalamus latiflorus. NEW FORESTS, 34, 177-186 |
| Lin CS*, Lin HL, Jane WN, Hsiao HW, Lin CC, Jheng FY, Chang WC* (2006) Changes in the morphology and cation content of a Bambusa edulis xylem mutant, vse, derived from somaclonal variation. JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 131(4), 445-451 |
| Lin CS*, Tseng MC, Hong PI, Chang WC* (2006) Albino inflorescence proliferation of Dendrocalamus latiflorus. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 42, 331-335 |
| Lin CS*, Lai YH, Sun CW, Liu NT, Tsay HS, Chang WC, Chen JJW* (2006) Identification of ESTs differentially expressed in green and albino mutant bamboo (Bambusa edulis) by suppressive subtractive hybridization (SSH) and microarray analysis. PLANT CELL TISSUE AND ORGAN CULTURE, 86(2), 169-175 |
| Lin CS*, Lin CC, Chang WC (2005) Shoot regeneration, re-flowering and post flowering survival in bamboo inflorescence culture. PLANT CELL TISSUE AND ORGAN CULTURE, 82, 243-249 |
| Lin CS*, Chen CT, Hsiao HW, Chang WC (2005) Effects of growth regulators on direct flowering of isolated ginseng buds in vitro. PLANT CELL TISSUE AND ORGAN CULTURE, 83, 241-244 |
| Lin CS*, Cheng MJ, Hsiao HW, Hong PI, Jheng FY, Lin CC, Chang WC (2005) Stamen-less inflorescence proliferation of Bambusa edulis. SCIENTIA HORTICULTURAE, 107, 76-80 |
| Lin CS*, Lin CC, Chang WC (2004) Effect of thidiazuron on vegetative tissue-derived somatic embryogenesis and flowering of bamboo Bambusa edulis. PLANT CELL TISSUE AND ORGAN CULTURE, 76, 75-82 |
| Chiu CC, Lin CS, Hsia AP, Su RC, Lin HL, Tsay YF* (2004) Mutation of a nitrate transporter, AtNRT1:4, results in a reduced petiole nitrate content and altered leaf development. Plant & Cell Physiology, 45(9), 1139-1148 |
| Lin CS*, Vidmar J, Chang WC (2004) Effects of growth regulators on inflorescence proliferation of Bamhusa edulis. PLANT GROWTH REGULATION, 43(3),221-225 |
| Lin CS*, Lin CC, Chang WC (2003) In vitro flowering of Bambusa edulis and subsequent plantlet survival. PLANT CELL TISSUE AND ORGAN CULTURE, 72, 71-78 |
| Lin CS*, Chen CT, Lin CC, Chang WC (2003) A method for inflorescence proliferation. Plant Cell Reports, 21(9), 838-843 |
| Hsu YH, Annamalai P, Lin CS, Chen YY, Chang WC, Lin NS* (2000) A sensitive method for detecting bamboo mosaic virus (BaMV) and establishment of BaMV-free meristem-tip cultures. PLANT PATHOLOGY, 49, 101-107 |
| Lin CS, Chang WC* (1998) Micropropagation of Bambusa edulis through nodal explants of field-grown culms and flowering of regenerated plantlets. Plant Cell Reports, 17, 617-620 |
Choun-Sea Lin
Senior Research Specialist
(02) 2787-2094
cslin99@gate.sinica.edu.tw
B101-2, White Building
Lab.
B101, White Building
Tel: (02) 2787-2094
1990 B.S. Dept. Horticulture, NCHU
1992 M.S. Dept. Horticutlure, NTU
1998 Ph. D. Dept. Horticulture, NTU
1998-2001 PDF Inst. of Mole. Biol., Academia Sinica
2001-2004 Assistant Prof., Asia University
2004-2007 Assistant Prof., Associate Prof., China Inst. of Tech.
2007-2010 Assistant Research Specialist, ABRC, Academia Sinica
2010-2015 Associate Research Specialist, ABRC, Academia Sinica
2015- Senior Research Specialist, ABRC, Academia Sinica