Development of an Orchid PLB Genetic Transformation and Micropropagation Platform

Protocorm-like bodies (PLBs) were successfully induced from flower stalk explants of the commercial Phalaenopsis cultivar V3 White, establishing a reliable platform for both clonal propagation and genetic transformation. This system preserves the original cultivar's genetic background and desirable horticultural traits while providing an efficient regeneration pathway. GUS reporter gene transformation and expression analyses have been conducted to optimize transformation efficiency and validate the platform. This technology provides an effective genetic improvement system for commercial orchid cultivars, enabling the introduction of genes associated with disease resistance, abiotic stress tolerance, and floral trait modification. The platform offers significant value for both fundamental research and commercial breeding applications, facilitating the development of elite orchid varieties with enhanced agronomic and ornamental characteristics.
Swee-Suak Ko