Breakthrough of wild tomato protoplast regeneration and DNA-free CRISPR-Cas9 genome editing.

Wild tomato (Solanum peruvianum) is an important genetic source for the tomato breeding but the progress has been hampered due to the self-incompatibility and highly heterozygous genome. Yao-Cheng Lin, Choun-Sea Lin and Ming-Che Shih from Agricultural Biotechnology Research Center developed a DNA-free CRISPR-Cas9 genome editing tool based on an optimized protoplast regeneration protocol. Cytogenetic analysis, whole genome sequencing and genome analysis confirms the stable and inheritable diploid and tetraploid regenerants. The study was published in Plant Physiology on April, 2022 and selected as the cover image for the April Issue. 

• Full text: https://doi.org/10.1093/plphys/kiac022  
• News and Views: https://doi.org/10.1093/plphys/kiac061  
• First author profile: https://plantae.org/choun-sea-lin-plant-physiology-first-author/
• Plant Physiology webinar highlighting Focus Issue on Gene Editing and its Applications:  https://youtu.be/wtD6105sfCE

Ming-Che Shih Yao-Cheng Lin Choun-Sea Lin

Choun-Sea Lin, Chen-Tran Hsu, Yu-Hsuan Yuan, Po-Xing Zheng, Fu-Hui Wu, Qiao-Wei Cheng, Yu-Lin Wu, Ting-Li Wu, Steven Lin, Jin-Jun Yue, Ying-Huey Cheng, Shu-I Lin, Ming-Che Shih, Jen Sheen, Yao-Cheng Lin^* (2022) Breakthrough of wild tomato protoplast regeneration and DNA-free CRISPR-Cas9 genome editing. Plant Physiology, Volume 188, Issue 4, April 2022, Pages 1917–1930