PHO2-Dependent Degradation of PHO1 Modulates Phosphate Homeostasis in Arabidopsis

The Arabidopsis pho2 mutant, which is defective in a ubiquitin-conjugating E2 enzyme, displays Pi toxicity as a result of enhanced uptake and root-to-shoot translocation of Pi To elucidate downstream components of the PHO2-dependent regulatory pathway, we identified two pho2 suppressors as carrying missense mutations in PHO1, which has been implicated in Pi loading to the xylem. In support of the genetic interaction between PHO1 and PHO2, we found that the protein level of PHO1 is increased in pho2, whereas such accumulation is ameliorated in both pho2 suppressors. Results from cycloheximide and endosomal cysteine protease inhibitor E-64d treatments further suggest that PHO1 degradation is PHO2-dependent and involves multivesicular body (MVB)-mediated vacuolar proteolysis. Using the transient expression system of tobacco leaves, we demonstrated that PHO1 and PHO2 are partially co-localized and physically interact in the endomembranes, where the ubiquitin conjugase activity of PHO2 is required for PHO1 degradation. In addition, reduced PHO1 expression caused by PHO1 mutations impede Pi uptake, indicating a functional association between xylem loading and acquisition of Pi. Together, our findings uncover a pivotal molecular mechanism by which PHO2 modulates the degradation of PHO1 in the endomembranes to maintain Pi homeostasis in plants.


Co-researchers:Tzu-Yin Liu, Teng-Kuei Huang, Ching-Ying Tseng, Ya-Shiuan Lai, Shu-I Lin, Wei-Yi Lin, June-Wei Chen