Goals and service content

Our ISH core facility provides plant tissue embedding and paraffin sectioning services. We are also actively developing new technologies to improve service quality. Hybrid-cut is developed by combining traditional paraffin sectioning and cryo-sectioning techniques for plant tissues with a harder texture and more crystals. The ISH core assists researchers in mapping the location of target genes in tissue cells.

RNA In Situ Hybridization (ISH)

Plant in situ hybridization (ISH) is a powerful technology that can detect and accurately locate target gene expression pattern in plant tissues. We have established stable tissue sectioning technology for Phalaenopsis, rice and Arabidopsis, and conduct RNA in situ hybridization technology to detect the gene expression site of target genes in plant cells. We are pleased that this ISH core service can assist researchers in their research.

References:

Ko, H.Y., Ho, L. H., Neuhaus,H. E., Guo, W. J. (2021). Transporter SlSWEET15 unloads sucrose from phloem and seed coat for fruit and seed development in tomato. Plant physiology 187  2230–2245 

Hsiao, A. S., Wang, K., & Ho, T. D. (2020). An Intrinsically Disordered Protein Interacts with the Cytoskeleton for Adaptive Root Growth under Stress. Plant physiology 183(2), 570–587.

Fang, S.C., Chen, J.C., and Wei, M.J. (2016). Protocorms and Protocorm-Like Bodies Are Molecularly Distinct from Zygotic Embryonic Tissues in Phalaenopsis aphrodite. Plant Physiol 171, 2682-2700.

Jang, S. (2015). Functional Characterization of PhapLEAFY, a FLORICAULA/LEAFY Ortholog in Phalaenopsis aphrodite. Plant Cell Physiol 56, 2234-2247.

Ko, S.S., Li, M.J., Ku, M.S.-B., Ho, Y.C., Lin, Y.J., Chuang, M.H., Hsing, H.X., Lien, Y.C., Yang, H.T., Chang, H.C., and Chan, M.T. (2014). The bHLH142 transcription factor coordinates with TDR1 to modulate the expression of EAT1 and regulate pollen development in rice. Plant Cell 26, 2486-2504.

Shih, M.C., Chou, M.L., Yue, J.J., Hsu, C.T., Chang, W.J., Ko, S.S., Liao, D.C., Huang, Y.T., Chen, J.J., Yuan, J.L., Gu, X.P., and Lin, C.S. (2014). BeMADS1 is a key to delivery MADSs into nucleus in reproductive tissues-De novo characterization of Bambusa edulis transcriptome and study of MADS genes in bamboo floral development. BMC Plant Biol 14, 179.