目標及服務內容

本核心實驗室提供植物組織包埋及石蠟切片服務，為提升服務品質，我們亦積極研發新技術。Hybrid-cut即為結合傳統石蠟切片與冷凍切片技術，針對質地較堅硬以及結晶較多的植物組織所研究出來的方法。

RNA原位雜合 (RNA In Situ Hybridization, ISH)

植物原位雜交技術是一個功能強大的技術，可偵測及精確定位出目標基因表達在組織部位。我們已經建立了穩定的蝴蝶蘭、水稻和阿拉伯芥的組織切片技術和RNA原位雜交技術檢測目標基因在組織細胞中表達的位置。我們很高興此ISH核心服務能協助研究人員的研究。

References:

Ko, H.Y., Ho, L. H., Neuhaus,H. E., Guo, W. J. (2021). Transporter SlSWEET15 unloads sucrose from phloem and seed coat for fruit and seed development in tomato. Plant physiology 187  2230–2245 

Hsiao, A. S., Wang, K., & Ho, T. D. (2020). An Intrinsically Disordered Protein Interacts with the Cytoskeleton for Adaptive Root Growth under Stress. Plant physiology 183(2), 570–587.

Fang, S.C., Chen, J.C., and Wei, M.J. (2016). Protocorms and Protocorm-Like Bodies Are Molecularly Distinct from Zygotic Embryonic Tissues in Phalaenopsis aphrodite. Plant Physiol 171, 2682-2700.

Jang, S. (2015). Functional Characterization of PhapLEAFY, a FLORICAULA/LEAFY Ortholog in Phalaenopsis aphrodite. Plant Cell Physiol 56, 2234-2247.

Ko, S.S., Li, M.J., Ku, M.S.-B., Ho, Y.C., Lin, Y.J., Chuang, M.H., Hsing, H.X., Lien, Y.C., Yang, H.T., Chang, H.C., and Chan, M.T. (2014). The bHLH142 transcription factor coordinates with TDR1 to modulate the expression of EAT1 and regulate pollen development in rice. Plant Cell 26, 2486-2504.

Shih, M.C., Chou, M.L., Yue, J.J., Hsu, C.T., Chang, W.J., Ko, S.S., Liao, D.C., Huang, Y.T., Chen, J.J., Yuan, J.L., Gu, X.P., and Lin, C.S. (2014). BeMADS1 is a key to delivery MADSs into nucleus in reproductive tissues-De novo characterization of Bambusa edulis transcriptome and study of MADS genes in bamboo floral development. BMC Plant Biol 14, 179.