A design of metal binding peptides: Proteomic survey of copper-binding domains in Arabidopsis roots
The root of plants is the frontier in metal ion uptake and therefore plays a crucial role in metal homeostasis. In this study, we conducted the first unbiased screening for copper-binding proteins in Arabidopsis roots. We examined the individual metalloproteomes of Cu and Zn in Arabidopsis roots on IMAC combined with MS. Of multiple Cu-IMAC-binding proteins in Arabidopsis roots visualized on 2-DE, 38 protein spots were identified by MS and further used in statistical analyses to reveal putative copper-binding motifs. These motifs will be referenced for enzyme modification and design of a novel gene-encoded peptide with specificity to heavy metal ions for phytoremediation in the future. Kung, C.-C., Huang, W.-N., Huang, Y.-C. and Yeh, K.-C. * (2006). Proteomic survey of copper-binding proteins in Arabidopsis roots by immobilized metal affinity chromatography and mass spectrometry. Proteomics, 6, pp.2746-2758 (Cover Illustration).Kuo-Chen Yeh
Distinguished Research Fellow and Director
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Director (2019.1.16-present)
Interim Director (2016.10.1 -2019.1.15)
Director/Coordinator of Molecular and Biological Agricultural Sciences program of Taiwan International Graduate Program (TIGP-MBAS), Academia Sinica (2015-2020)
Distinguished Research Fellow (2024-present)
Research Fellow (2014-2024)
Associate Research Fellow (2009-2013)
Assistant Research Fellow (2001-2009)
PDF Howard Hughes Medical Institute and Department of Biological Sciences, Stanford University, USA (1999-2001)
Ph.D. Plant Biology Graduate Group, University of California, Davis, USA (1994-1999)