目標及服務內容

本核心實驗室提供植物組織包埋及石蠟切片服務，為提升服務品質，我們亦積極研發新技術。Hybrid-cut即為結合傳統石蠟切片與冷凍切片技術，針對質地較堅硬以及結晶較多的植物組織所研究出來的方法。

RNA原位雜合 (RNA In Situ Hybridization, ISH)

植物原位雜交技術是一個功能強大的技術，可偵測及精確定位出目標基因表達在組織部位。我們已經建立了穩定的蝴蝶蘭、水稻和阿拉伯芥的組織切片技術和RNA原位雜交技術檢測目標基因在組織細胞中表達的位置。我們很高興此ISH核心服務能協助研究人員的研究。

References:

Ko, S.S., Li, M.J., Ho, Y.C., Yu, C.P., Yang, T.T., Lin, Y.J., Hsing, H.C., Chen, T.K., Jhong, C.M., Li, W.H., Sun-Ben, Ku.M. (2021) Rice transcription factor GAMYB modulates bHLH142 and is homeostatically regulated by TDR during anther tapetal and pollen development. J Exp Bot 72 (13) 4888-4903

Ko, H.Y., Ho, L. H., Neuhaus,H. E., Guo, W. J. (2021). Transporter SlSWEET15 unloads sucrose from phloem and seed coat for fruit and seed development in tomato. Plant physiology 187  2230–2245 

Hsiao, A. S., Wang, K., & Ho, T. D. (2020). An Intrinsically Disordered Protein Interacts with the Cytoskeleton for Adaptive Root Growth under Stress. Plant physiology 183(2), 570–587.

Lin, Y.J., Li, M.J., Hsing, H.C., Chien, T.K., Yang, T.T., Ko, S.S. (2019) Spike Activator 1, Encoding a bHLH, Mediates Axillary Bud Development and Spike Initiation in Phalaenopsis aphrodite. Int J Mol Sci, 20(21)

Fang, S.C., Chen, J.C., and Wei, M.J. (2016). Protocorms and Protocorm-Like Bodies Are Molecularly Distinct from Zygotic Embryonic Tissues in Phalaenopsis aphrodite. Plant Physiol 171, 2682-2700.

Jang, S. (2015). Functional Characterization of PhapLEAFY, a FLORICAULA/LEAFY Ortholog in Phalaenopsis aphrodite. Plant Cell Physiol 56, 2234-2247.

Ko, S.S., Li, M.J., Ku, M.S.-B., Ho, Y.C., Lin, Y.J., Chuang, M.H., Hsing, H.X., Lien, Y.C., Yang, H.T., Chang, H.C., and Chan, M.T. (2014). The bHLH142 transcription factor coordinates with TDR1 to modulate the expression of EAT1 and regulate pollen development in rice. Plant Cell 26, 2486-2504.

Shih, M.C., Chou, M.L., Yue, J.J., Hsu, C.T., Chang, W.J., Ko, S.S., Liao, D.C., Huang, Y.T., Chen, J.J., Yuan, J.L., Gu, X.P., and Lin, C.S. (2014). BeMADS1 is a key to delivery MADSs into nucleus in reproductive tissues-De novo characterization of Bambusa edulis transcriptome and study of MADS genes in bamboo floral development. BMC Plant Biol 14, 179.