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演講訊息

草莓(豐香品系)中兩個熱休克轉錄因子同源基因與植株耐熱性間的關聯性

Conference Room A133, Agricultural Technology Building
2016/01/18 10:30 AM
Dr. Po-Hsuan Chou (Associate Professor, Department of Life Sciences, Tzu-Chi University, Taiwan)
Host: Choun-Sea Lin


TBA

Auditorium A134, Agricultural Technology Building
2016/01/21 10:30 AM
Dr. Kyaw Aung (Post-doctoral Research Fellow, Howard Hughes Medical Institute, Michigan State University, USA)
Host: Tzyy-Jen Chiou


A bacterial effector targets host plasmodesmata to promote pathogen virulence in plants

Auditorium A134, Agricultural Technology Building
2016/01/21 10:30 AM
Dr. Kyaw Aung (Post-doctoral Research Fellow, Howard Hughes Medical Institute, Michigan State University, USA)
Host: Tzyy-Jen Chiou
The Arabidopsis–Pseudomonas syringae pv. tomato (Pst) DC3000 pathosystem has been extensively studied to elucidate basic principles underlying plant–microbe interaction; however, little is known whether bacteria modulate adjoining non-infected plant cells for successful infection. Recent reports demonstrated that plants induce plasmodesmatal closure as part of a defense response against bacterial infection. Pst DC3000 injects 36 virulence effector proteins into plant cells to subvert plant immunity. We discovered that Pst DC3000 effector HopO1-1 is targeted to the plasmodesmata in Arabidopsis. Expression of HopO1-1 in Arabidopsis leads to an increase in PD-dependent molecular flux between plant cells. Moreover, HopO1-1 is physically associated with PD-located protein 5 (PDLP5), which is previously shown to be involved in plant immunity, and PDLP7. Consistent with its predicted function, we demonstrated that HopO1-1 is an active ADP ribosyltransferase enzyme. Our results suggest that bacterial pathogens deliver effectors such as HopO1-1 to modulate host plasmodesmatal function, presumably by ADP-ribosylating PDLPs, to facilitate bacterial spread and multiplication.


Plant Translational Research in TNT Bioremediation

Conference Room A133, Agricultural Technology Building
2016/01/25 10:30 AM
Dr. Duen-Wei Hsu (Assistant Professor, Department of Biotechnology, National Kaohsiung Normal University, Taiwan)
Host: Hsin-Hung Yeh


Molecular Imaging of Proteins in Biological Samples Using Ambient Ionization Mass Spectrometry

Auditorium A134, Agricultural Technology Building
2016/02/01 10:30 AM
Dr. Cheng-Chih Hsu (Assistant Professor, Department of Chemistry, National Taiwan University)
Host: Yu-Liang Yang
Mass spectrometric imaging (MSI) is an emerging tool for biological research. Matrix-assisted laser desroption ioniization (MALDI) imaging is the most widely used MSI technology at present especially for protein imaging. However its utility is limited for pathological investigation due to the use of organic matrix. So-called matrix-free molecular imaging at atmospheric pressure has been developed to bypass this barrier. Molecular imaging of lipids and metabolites in tissues under ambient conditions is readily achieved using desorption electrospray ionization (DESI), but DESI is essentially blind to the presence of proteins. In 2013 we demonstrated that the use of nanospray desorption electrospray ionization (nanoDESI) in combination with an inverted microscope overcomes this problem and allows us to visualize proteins in tissue samples in a label-free manner at atmospheric pressure with only minimum sample preparation. However this microscopy-guided approach is not efficient especially when imaging larger tissue sections. To improve the utility of this technique, we interfaced a 2D stage with nanoDESI for direct and continuous mass spectrometry scannings on tissue sections. Multiply charged proteins with masses up to 15 kDa were successfully measured by nanoDESI using an LTQ Orbitrap mass spectrometers. In a mice brain section, expression of proteins previously found by matrix-assisted laser desroption ioniization (MALDI) mass spectrometry imaging, including ubiquitin, -thymosin, and myelin basic protein, were spatially-mapped, and the identified via top-down approaches. We also determined the location of methylation on myelin basic protein. This imaging modality was further implemented to MYC-induced lymphomas. We observed an array of truncated proteins in the region where normal thymus cells were infiltrated by tumor cells, in contrast to healthy tissue. The detection limits for cytochrome c and insulin are 10-50 picomoles per mg of tissue, at which level many endogenous proteins are expressed in cells. It is expected that this new tool will provide important biological insight into cellular processes.


My buzz life

Auditorium A134, Agricultural Technology Building
2016/02/22 10:30 AM
Dr. Yue-Wen Chen (Professor, Department of Biotechnology and Animal Science, National Ilan University)
Host: Yu-Liang Yang


Identification of plant vacuolar transporters mediating phosphate storage

Auditorium A134, Agricultural Technology Building, Academia Sinica
2016/03/21 10:30 AM
Mr. Teng-Kuei Huang
Host: Dr. Tzyy-Jen Chiou


My Personal Experience in R&D of Biotechnology: 20 years in USA and 20 Years in Taiwan

Auditorium A134, Agricultural Technology Building
2016/03/28 10:30 AM
Dr. Ning-Sun Yang (Distinguished Research Fellow, Agricultural Biotechnology Research Center, Academia Sinica)
Host: Tzyy-Jen Chiou


The role of fusion in mitochondrial quality control

Auditorium A134, Agricultural Technology Building
2016/05/09 10:30 AM
Dr. Wei Yuan Yang (Associate Research Fellow, Institute of Biological Chemistry, Academia Sinica)
Host: Der-Fen Suen


The metal hyperaccumulator Arabidopsis halleri - a model for plant metal homeostasis and the adaptation to extreme environments

Auditorium A134, Agricultural Technology Building
2016/05/19 10:30 AM
Dr. Stephan Clemens (Professor, Department of Plant Physiology, University of Bayreuth, Germany)
Host: Kuo-Chen Yeh


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