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Development of new cytokinin derivatives for plant biotechnology: from synthesis to analysis and back again

Auditorium A134, Agricultural Technology Building, Agricultural Biotechnology Research Center
2018/03/05 10:30 AM
Dr. Karel Doležal (Senior Researcher, Head of Chemical Biology and Genetics Department, Centre of the Region Haná for Biotechnological and Agricultural Research, the Czech Republic)
Host: Su-Chiung Fang
Cytokinins (CK) are an important class of plant hormones, organic substances naturally occurring at extremely low concentrations, which regulate major physiological processes in plants.
6-Benzylaminopurine (BAP), an important and affordable cytokinin, is routinely utilized in micropropagation for its effective stimulatory properties. However, this compound and/or its endogenous metabolites may negatively influence the shoot proliferation, rooting and acclimatization processes in some plant species (Werbrouck et al., 1996; Strnad, 1997). Therefore, the development of other new CK derivatives exhibiting high morphogenetic activity might consequently be of a great practical importance in plant biotechnology. Based on our search for naturally occurring aromatic cytokinins in plants, we recently synthesized several new groups of their analogues having high activity in different CK bioassays as well as the ability to activate cytokinin receptors and/or to inhibit CKX. The best compounds have been tested as a new tool for retardation of senescence during the multiplication stage of micropropagation of selected plant species as well as to support rooting and acclimatization competence. Subsequently, wide range of endogenous plant hormones and their metabolites were quantified during these experiments and compared in relation to cytokinin exogenously used as well as micropropagation efficiency. Results of these measurements have been used to design second generation of 9-substituted CK derivatives with improved metabolic properties.

Research was supported by the Ministry of Education Youth and Sports, Czech Republic
(grant LO1204 from the National Program of Sustainability and Agricultural Research).


Biochemical and Functional Characterization of Seed Coat Mucilage

Auditorium A134, Agricultural Technology Building, Agricultural Biotechnology Research Center
2018/03/12 10:00 AM
Dr. Allen Yi-Lun Tsai (Post-doctoral Fellow, Graduate School of Science and Technology, Kumamoto University, Japan)
Host: Hsin-Hung Yeh


Function of alternatively spliced isoforms and a small peptide in root development

Auditorium A134, Agricultural Technology Building, Agricultural Biotechnology Research Center
2018/03/19 10:00 AM
Dr. Masashi Yamada (Postdoctoral Fellow, Duke University, U.S.A.)
Host: Yet-Ran Chen


  

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