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演講訊息

我的生技旅程: 感染症疫苗開發

Auditorium A134, Agricultural Technology Building, Academia Sinica
2019/02/25 10:30 AM
Dr. Juine-Ruey Chen (RuenHuei Biopharmaceuticals. Inc.)
Host: 楊聿智先生 (蕭培文實驗室)


Cellular growth regulation in roots - how to adapt in a complex environment

Auditorium A134, Agricultural Technology Building, Agricultural Biotechnology Research Center
2019/03/04 10:30 AM
Dr. Guido Grossmann (Group Leader, the Centre for Organismal Studies (COS) Heidelberg, Heidelberg University, Germany)
Host: Cheng-Hsun Ho
In nature, plant roots grow in highly heterogeneous conditions, which requires both systemic and local response mechanisms to modulate cellular growth and adapt organ morphology. How environmental cues are integrated into growth regulation and how systemic communication and cell-autonomous responses are interlinked in this process, remain challenging questions in plant biology. To facilitate the investigation of root-environment interactions, we combine the use microfluidic lab-on-a-chip platforms with live-cell imaging of roots. Over the past years, we developed a series of microfluidic devices for on-chip root growth under precisely controlled conditions. Here, we present the dual-flow-RootChip, a microfluidic organ-on-a-chip platform for asymmetric perfusion of Arabidopsis roots to investigate root growth, development, and signaling under simulated environmental heterogeneity. Applications range from monitoring physiology and signaling (e.g. calcium sign.) under asymmetric conditions, tracing molecular uptake and selective drug treatments to local infection or colonization by fungi or bacteria.
Using the dual-flow-RootChip, we revealed cell-autonomous regulation of root hair growth upon differential availability of inorganic phosphate (Pi). Independent of the overall Pi status of the root, we found that hair cells can triple their growth rate within minutes, when extracellular Pi levels rise, pointing to a direct modulation of the tip growth machinery and resulting in hair growth that followed the nutrient gradient.
To understand the molecular mechanisms of growth regulation in root hairs, we further dissected the tip growth machinery and unveiled a phased assembly process during the early stages of hair emergence. The transition between different phases depends on timed regulation of Rho of plants (ROP) GTPases. We identified a novel candidate that acts upstream of ROPs, pre-forms a polar domain at the plasma membrane, and is necessary and
sufficient to recruit ROPs in order to trigger the initiation of tip growth.


Mechanism of Agrobacterium T-DNA integration into the plant genome

Auditorium A134, Agricultural Technology Building
2019/03/13 10:30 AM
Dr. Stanton Bruce Gelvin (H. Edwin Umbarger Distinguished Professor, Department of Biological Sciences, Purdue University, USA)
Host: Choun-Sea Lin
The mechanism of T-DNA integration into the plant genome remains controversial. Because T-DNAs containing sequences homologous to the plant genome rarely integrate using homologous recombination, it has been assumed that a non-homologous end-joining (NHEJ) pathway is used for integration. Although some studies have indicated less stable transformation of Arabidopsis or rice NHEJ mutants compared to wild-type plants, our laboratory has shown that single or higher order mutants in both the classical (cNHEJ) pathway and the alternative NHEJ pathway (using microhomology-mediated end-joining; MMEJ) maintain stable transformation frequencies similar to or even greater than that of wild-type plants. These transformation results were confirmed by blotting of high molecular weight DNA from transformed but not selected plant tissues, and by suppression PCR analysis of T-DNA/plant DNA junctions from these tissues. We hypothesize that delayed repair of double-strand DNA breaks in these mutants provides more opportunity for T-DNA integration. Recently, the Hooykaas laboratory proposed that DNA polymerase theta (PolQ) is required for T-DNA integration into the Arabidopsis genome. We have examined both Arabidopsis tebichi (teb/polQ) and rice CRISPR-generated polQ mutants for transient and stable Agrobacterium-mediated transformation. These mutants show decreased transient transformation but significant (~20% wild-type) levels of stable transformation. T-DNA/plant DNA junctions isolated from stably transformed rice and Arabidopsis tissues are similar to those isolated from wild-type plants. Both rice and Arabidopsis polQ mutants show growth/developmental phenotypes that may be responsible for decreased transformation frequency: Arabidopsis polQ mutant root calli grow slowly, and rice polQ mutant calli fail to regenerate plants. Taken together, these results indicate either that these various DNA repair pathways function redundantly, or that some unknown pathway is used for T-DNA integration into the plant genome.


The multifunctional nucleole: more than ribosome biogenesis

Auditorium A134, Agricultural Technology Building
2019/03/27 11:00 AM
Dr. Julio Sáez-Vásquez (Professor, Laboratory of Genomes and Plant Development, University of Perpignan, France)
Host: Tzyy-Jen Chiou


Drug development for disease treatment

Auditorium A134, Agricultural Technology Building
2019/04/08 10:00 AM
Dr. Taina Wu (Research Fellow, MICROBIO CO., LTD.)
Host: Yu-Liang Yang


Targeting Myeloid Suppressive Cells as a Promising Cancer Immunotherapy

Auditorium A134, Agricultural Technology Building
2019/04/10 10:00 AM
Dr. Hui-Ming Chen (Assistant Member, Houston Methodist Research Institute)
Host: Yu-Ling Lin


Genetic Structure Reveals the Behavior of Natural Arabidopsis Accessions in Response to Limited Phosphate

Auditorium A134, Agricultural Technology Building
2019/04/29 10:30 AM
Dr. Pei-Shan Chien (Dr. Tzyy-Jen Chiou's Lab)
Host: Tzyy-Jen Chiou


Roles of Allelochemicals in Ecosystem Complexity and Agricultural Biotechnology

農業科技大樓1樓A134演講廳
2019/05/13 10:30 AM
周昌弘院士(中央研究院植物暨微生物學研究所通信研究員/中央研究院院士)
Host: 葉信宏


Synthetic-Genetic-Array Based Yeast One-Hybrid System for the Construction of Gene Regulatory Network in Wood Formation

Auditorium A134, Agricultural Technology Building
2019/05/27 10:30 AM
Dr. Ying-Chung Jimmy Lin (Assistant Professor, Department of Life Science, National Taiwan University)
Host: 陳盈嵐


台灣番茄育種與採種

農業科技大樓1樓A133會議室
2019/06/12 10:30 AM
陳迪偉博士(瑞成種苗有限公司)
Host: 林崇熙


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