Orchid Transformation Core Facility
Chii-Gong Tong / Research Assistant Specialist
2006-present Research Assistant Specialist Agricultural Biotechnology Research Center, Academia Sinica
2002-2004 Senior Scientist & Director of RD CytoPharm,Taiwan
1999-2002 Assistant Professor Dept. Life Sci. Natl. Central Univ.
1995-1999 PDF MCDB Institute, University of Texas at Austin, USA.
1995 Ph.D. Biological Sciences , University of Texas at Austin, USA.
Tel: (06) 505-6630#310 (Office), (06) 505-6630#303 (Lab)
Service and Technology Development
Although tons of efforts were polled in last decade, orchid remains as tough and difficult targets for genetic transformation. Only few publications that documented orchid transformation were reported on Phalaenopsis, Oncidium and Dendorbium species, yet the genetic engineered orchids have not even been close to commercialization. The Orchidaceae is monocot plants that are normally slow in cell division which confers low regeneration speed in last stage of micropropagation. Secretive toxic phenolics hamper growth and differentiation on explants, which make the transformation diffcult tasks. Thus, the tissue culture methods usually lead to extremely low rate of transformation and extra long time to the successes. Currently, using Phalaenopsis orchids, we are developing the pollen mediated transformation platforms that should reduce the time of generating transformant seedlings by more than an half. The pollen platforms almost eliminate tissue culture facility that may result in saving cost for the R&D or orchid industry. In this stage, this core is still under preparation. We expect this core facility can provide a new and more effcient orchid transformation technology service to the researchers of Academia Sinica or the orchid industry in Taiwan.