 HIGHLIGHTS  Kuo, H.-F., Hsu, Y.-Y., Lin, W.-C., Chen, K.-Y., Munnik, T., Brearley, C. A.* and Chiou, T.-J.* (2018) Arabidopsis inositol phosphate kinases, IPK1 and ITPK1, constitute a metabolic pathway in maintaining phosphate homeostasis. The Plant J. ( doi:10.1111/tpj.13974)  Emerging studies have implicated a close link between inositol phosphate (InsP) metabolism and cellular phosphate (Pi) homeostasis in eukaryotes; however, whether a common InsP species is deployed as an evolutionarily conserved metabolic messenger to mediate Pi signaling remains unknown. Here, using genetics and InsP profiling combined with Pi starvation response (PSR) analysis in Arabidopsis thaliana, we showed that the kinase activity of inositol pentakisphosphate 2-kinase (IPK1), an enzyme required for phytate (inositol hexakisphosphates; InsP6) synthesis, is indispensable for maintaining Pi homeostasis under Pi-replete conditions, and inositol 1,3,4-trisphosphate 5/6-kinase 1 (ITPK1) plays an equivalent role. Although both ipk1-1 and itpk1 mutants exhibited decreased levels of InsP6 and diphosphoinositol pentakisphosphate (PP-InsP5; InsP7), disruption of another ITPK family enzyme, ITPK4, which correspondingly caused depletion of InsP6 and InsP7, did not display similar Pi-related phenotypes, which precludes these InsP species as effectors. Notably, the level of D/L-Ins(3,4,5,6) P4 was concurrently elevated in both ipk1-1 and itpk1 mutants, which showed a specific correlation to the misregulated Pi phenotypes. However, the level of D/L-Ins(3,4,5,6)P4 is not responsive to Pi starvation that instead manifests a shoot-specific increase in InsP7 level. This study demonstrates a more nuanced picture of the intersection of InsP metabolism and Pi homeostasis and PSR than has previously been elaborated and additionally establishes intermediate steps to phytate biosynthesis in plant vegetative tissues. link
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Frontiers in BioAgricultural Research: The 20th Anniversary of the Agricultural Biotechnology Research Center, Academia Sinica
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 PHOTOS
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 SEMINAR
 2018/10/18 10:30 AM
Dr. Simon Gilroy (Professor, Department of Botany, University of Wisconsin, USA)
Life without gravity: Plant stress biology on the International Space Station
Auditorium A134, Agricultural Technology Building, Agricultural Biotechnology Research Center 2018/10/19 9:00 AM~9:45 AM
Dr. Kuo-Chen Yeh (Research Fellow and Interim Director, Agricultural Biotechnology Research Center, Academia Sinica)
Heavy metal homeostasis in plants and applications
Auditorium A134, Agricultural Technology Building, Agricultural Biotechnology Research Center
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