Yue JJ, Hong CY, Wei P, Tsai YC, Lin CS (2020) How to start your monocot CRISPR/Cas project: plasmid design, efficiency detection, and offspring analysis. Rice13:9.
The breakthrough CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9-mediated genome-editing technology has led to great progress in monocot research; however, several factors need to be considered for the efficient implementation of this technology. To generate genome-edited crops, single guide (sg)RNA and Cas9 DNA are delivered into plant cells and expressed, and the predicted position is targeted. Analyses of successful targeted mutations have revealed that the expression levels, expression timing, and variants of both sgRNA and Cas9 need to be sophisticatedly regulated; therefore, the promoters of these genes and the target site positions are the key factors for genome-editing efficiency. Currently, various vectors and online tools are available to aid sgRNA design. Furthermore, to reduce the sequence limitation of the protospacer adjacent motif (PAM) and for other purposes, many Cas protein variants and base editors can be used in plants. Before the stable transformation of a plant, the evaluation of vectors and target sites is therefore very important. Moreover, the delivery of Cas9-sgRNA ribonucleoproteins (RNPs) is one strategy that can be used to prevent transgene issues with the expression of sgRNA and Cas proteins. RNPs can be used to efficiently generate transgene-free genome-edited crops that can reduce transgene issues related to the generation of genetically modified organisms. In this review, we introduce new techniques for genome editing and identifying marker-free genome-edited mutants in monocot crops. Four topics are covered: the design and construction of plasmids for genome editing in monocots; alternatives to SpCas9; protoplasts and CRISPR; and screening for marker-free CRISPR/Cas9-induced mutants. We have aimed to encompass a full spectrum of information for genome editing in monocot crops.
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2020/04/13 11:00 AM
Dr. Julian Schroeder (Novartis Distinguished Professor in Plant Sciences, University of California, San Diego, USA) CO2 Sensing in Plants and New Insights into Drought-Induced Abscisic Acid Signal Transduction Auditorium A134, Agricultural Technology Building 2020/09/07 10:00 AM
Dr. Michael Alan Huffman (Associate Professor (tenured), Department of Ecology and Social Behavior, Primate Research Institute, Kyoto University, Japan) Learn from primate self-medication about the maintenance of human and domestic animal health? Auditorium A134, Agricultural Technology Building
Yuh Tzean, Ho-Hsiung Chang, Tsui-Chin Tu, Bo-Han Hou, Ho-Ming Chen, Yi-Shu Chiu, Wei-Yi Chou, Li Chang, and Hsin-Hung Yeh* (2020) Engineering Plant Resistance to Tomato Yellow Leaf Curl Thailand Virus Using a Phloem-Specific Promoter Expressing Hairpin RNA https://apsjournals.apsnet.org/doi/10.1094/MPMI-06-19-0158-R (Selected for publication in MPMI Focus Issue on Cell Biology of Virus-Plant and Virus-Vector Interaction and commentary in the same issue).
S. Kailasam, S. Singh, M.-J. Liu, C.-C. Lin and K.-C. Yeh* (2020) A HemK class glutamine-methyltransferase is involved in the termination of translation and essential for iron homeostasis in Arabidopsis. New Phytologistdoi.org/10.1111/nph.16440
Yamada M, Han X, Benfey PN (2020) RGF1 controls root meristem size through ROS signaling. Nature. 2020 Jan;577(7788):85-88. doi: 10.1038/s41586-019-1819-6. Epub 2019 Dec 4.
H.-F. Chang, S.-L. Wang*, D.-C. Lee, S. S.-Y. Hsiao, Y. Hashimoto, and K.-C. Yeh* (2020) Assessment of indium toxicity to the model plant Arabidopsis. Journal of Hazardous Materialsdoi.org/10.1016/j.jhazmat.2019.121983
Transgenic approaches employing RNA interference (RNAi) strategies have been successfully applied to generate desired traits in plants; however, variations between RNAi transgenic siblings and the ability to quickly apply RNAi resistance to diverse cultivars remain challenging. ...more
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