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  • Applications for 2021 admission to the AS-NTU PhD Program 2020/09/23
  • Epidemic prevention 2020/03/20

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Yen-Ling Lin, Chin-Lin Chung, Ming-Hui Chen, Chun-Han Chen, Su-Chiung Fang* (2020) SUMO protease SMT7 modulates ribosomal protein L30 to regulate cell-size checkpoint function. Plant Cell. 32(4): 1285-1307.
Proliferating cells actively coordinate growth and cell division to ensure cell-size homeostasis; however, the underlying mechanism through which size is controlled is poorly understood. Defect in a SUMO protease protein, suppressor of mat3 7 (SMT7), has been shown to reduce cell division number and increase cell size of the small-size mutant mating type locus 3-4 (mat3-4), which contains a defective retinoblastoma tumor suppressor-related protein of Chlamydomonas (Chlamydomonas reinhardtii). Here we describe development of an in vitro SUMOylation system using Chlamydomonas components and use it to provide evidence that SMT7 is a bona fide SUMO protease. We further demonstrate that the SUMO protease activity is required for supernumerous mitotic divisions of the mat3-4 cells. In addition, we identified RIBOSOMAL PROTEIN L30 (RPL30) as a prime SMT7 target and demonstrated that its SUMOylation is an important modulator of cell division in mat3-4 cells. Loss of SMT7 caused elevated SUMOylated RPL30 levels. Importantly, overexpression of the translational fusion version of RPL30-SUMO4, which mimics elevation of the SUMOylated RPL30 protein in mat3-4, caused a decrease in mitotic division and recapitulated the size-increasing phenotype of the smt7-1 mat3-4 cells. In summary, our study reveals a novel mechanism through which a SUMO protease regulates cell division in the mat3-4 mutant of Chlamydomonas and provides yet another important example of the role that protein SUMOylation can play in regulating key cellular processes, including cell division.

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2020/08/24 ABRC Seminar

2020/08/17 ABRC Seminar 2020/02/24 ABRC Seminar 2020/03/09  ABRC Seminar

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*2020/09/28 11:00 AM
鍾尊智執行長 (奈捷生物科技股份有限公司執行長暨共同創辦人)
奈捷光感測技術(FOPPR)在生物標記(Biomarker)從研究端使用到臨床與應用
Auditorium A134, Agricultural Technology Building

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Size control study of microalga reveals secret of aberrant cell division!

Apaya MK, Hsiao PW, Yang YC and Shyur LF* (2020). Deregulating the CYP2C19/epoxy-eicosatrienoic acid-associated FABP4/FABP5 signaling network as a therapeutic approach for metastatic triple-negative breast cancer Cancers 12(1):199. doi: 10.3390/cancers12010199.

Wen-Chi Lee, Bo-Han Hou, Cheng-Yu Hou, Shu-Ming Tsao, Ping Kao, Ho-Ming Chen* (2020) Widespread Exon Junction Complex Footprints in the RNA Degradome Mark mRNA Degradation before Steady State Translation. The Plant Cell, Vol. 32: 904–922.

Crime scene reconstruction: determining time of RNA death by analysis of RNA degradation fragments

Yue JJ, Hong CY, Wei P, Tsai YC, Lin CS (2020) How to start your monocot CRISPR/Cas project: plasmid design, efficiency detection, and offspring analysis. Rice 13:9.

Retinoblastoma (RB) is the first identified tumor suppressor. Mutation in RB not only causes retinoblastoma, its mutation is also associated with many types of cancer. Hence, it is important to study how the RB pathway regulates cell division. Unfortunately, RB null mutants often lead to embryonic lethality in mammalian model systems such as mouse. ...more
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