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Cindy Ast, Jessica Foret, Luke M. Oltrogge, Roberto De Michele, Thomas J. Kleist, Cheng-Hsun Ho & Wolf B. Frommer(2017) Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins.Nature Communications(2017), doi:10.1038/s41467-017-00400-2
Sensitivity, dynamic and detection range as well as exclusion of expression and instrumental artifacts are critical for the quantitation of data obtained with fluorescent protein (FP)-based biosensors in vivo. Current biosensors designs are, in general, unable to simultaneously meet all these criteria. Here, we describe a generalizable platform to create dual-FP biosensors with large dynamic ranges by employing a single FP-cassette, named GO-(Green-Orange) Matryoshka. The cassette nests a stable reference FP (large Stokes shift LSSmOrange) within a reporter FP (circularly permuted green FP). GO- Matryoshka yields green and orange fluorescence upon blue excitation. As proof of concept, we converted existing, single-emission biosensors into a series of ratiometric calcium sensors (MatryoshCaMP6s) and ammonium transport activity sensors (AmTryoshka1;3). We additionally identified the internal acid-base equilibrium as a key determinant of the GCaMP dynamic range. Matryoshka technology promises flexibility in the design of a wide spectrum of ratiometric biosensors and expanded in vivo applications. link

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*2017/12/18 10:30 AM
Dr. Anudep Rungsipipat (Associate Professor, Companion Animal Cancer Research Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand)
Chemopreventive Effects of Thai Herbs on Mice Induced Colorectal Carcinogenesis
Auditorium A134, Agricultural Technology Building, Agricultural Biotechnology Research Center

*2017/12/22 10:30 AM
Hen-Hong Chang (Dean, School of Chinese Medicine, Distinguished Professor, China Medical University, Taiwan)
Herb, food and constitution in Traditional Chinese Medicine
Auditorium A134, Agricultural Technology Building, Agricultural Biotechnology Research Center

*2017/12/25 10:30 AM
Dr. Tsong-Long Hwang (Dean/Distinguished Professor, College of Human Ecology, Chang Gung University of Science and Technology, Taiwan)
Research on traditional herbal medicines for neutrophilic inflammatory diseases
Auditorium A134, Agricultural Technology Building, Agricultural Biotechnology Research Center

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Chin-Hsien Tsai, Sheue-Fen Tzeng, Shih-Chuan Hsieh, Yu-Chih Yang, Yi-Wen Hsiao, Mong-Hsun Tsai, and Pei-Wen Hsiao (2017) A standardized herbal extract mitigates tumor inflammation and augments chemotherapy effect of docetaxel in prostate cancer. Scientific Reports 2017 Nov; 7: 15624

Jeng-Yuan Shiau, Kyoko Nakagawa-Goto, Kuo-Hsiung Lee, and Lie-Fen Shyur* (2017) Phytoagent deoxyelephantopin derivative inhibits triple negative breast cancer cell activity by inducing oxidative stress-mediated paraptosis-like cell death. Oncotarget, 2017, 8:56942-56958

Cheng-Hsun Ho* and Wolf B. Frommer (2016) Design and Functional Analysis of Fluorescent Nitrate and Peptide Transporter Acativity Sensors in Yeast Cultures Bio-protocol, vol 6, iss 3

Chin-Hsien Tsai, Sheue-Fen Tzeng, Shih-Chuan Hsieh, Chia-Jui Tsai, Yu-Chih Yang, Mong-Hsun Tsai and Pei-Wen Hsiao A Standardized Wedelia chinensis Extract Overcomes the Feedback Activation of HER2/3 Signaling upon Androgen-Ablation in Prostate Cancer. Frontiers in Pharmacology 2017 Oct; 8:721

Ji-Rong Yang, Chieh-Yu Cheng, Chih-Yuan Chen, Chao-Hua Lin, Chuan-Yi Kuo, Hsiang-Yi Huang, Fu-Ting Wu, Yu-Chih Yang, Chia-Ying Wu, Ming-Tsan Liu*, and Pei-Wen Hsiao* (2017) A virus-like particle vaccination strategy expands its tolerance to H3N2 antigenic drift by enhancing neutralizing antibodies against hemagglutinin stalk. Antiviral Research, 2017, 140: 62-75

Docetaxel remains the standard treatment for castration-resistant prostate cancer by improving overall survival but having severe adverse toxicity. Inflammatory cytokines in the tumor microenvironment promote docetaxel resistance through activation of IKKα/β-NFκB pathways. ...more
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