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Choun-Sea Lin*,Chen-Tran Hsu, Ling-Hung Yang, Lan-Ying Lee, Jin-Yuan Fu, Qiao-Wei Cheng, Fu-Hui Wu, Han Chen-Wei Hsiao, Yesheng Zhang, Ru Zhang, Wan-Jung Chang, Chen-Ting Yu, Wen Wang, Li-Jen Liao, Stanton B. Gelvin*,Ming-Che Shih*. (2018) Application of protoplast technology to CRISPR/Cas9 mutagenesis: From single cell mutation detection to mutant plant regeneration. Plant Biotechnol J. 2017 Dec 12. doi: 10.1111/pbi.12870
Plant protoplasts are useful for assessing the efficiency of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR Associated Protein 9 (Cas9) mutagenesis. We improved the process of protoplast isolation and transfection of several plant species. We also developed a method to isolate and regenerate single mutagenized Nicotianna tabacum protoplasts into mature plants. Following transfection of protoplasts with constructs encoding Cas9 and sgRNAs, target gene DNA could be amplified for further analysis to determine mutagenesis efficiency. We investigated N. tabacum protoplasts and derived regenerated plants for targeted mutagenesis of the phytoene desaturase (NtPDS) gene. Genotyping of albino regenerants indicated that all four NtPDS alleles were mutated in amphidiploid tobacco, and no Cas9 DNA could be detected in most regenerated plants. link

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The visiting of Asia-Pacific Association of Agricultural Research Institutions, APAARI

2017/01/22 ABRC Seminar 2018/01/15 ABRC Seminar 2018/01/12 ABRC Seminar

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*2018/03/05 10:30 AM
Dr. Karel Doležal (Senior Researcher, Head of Chemical Biology and Genetics Department, Centre of the Region Haná for Biotechnological and Agricultural Research, the Czech Republic)
Development of new cytokinin derivatives for plant biotechnology: from synthesis to analysis and back again
Auditorium A134, Agricultural Technology Building, Agricultural Biotechnology Research Center

*2018/03/12 10:00 AM
Dr. Allen Yi-Lun Tsai (Post-doctoral Fellow, Graduate School of Science and Technology, Kumamoto University, Japan)
Biochemical and Functional Characterization of Seed Coat Mucilage
Auditorium A134, Agricultural Technology Building, Agricultural Biotechnology Research Center

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ADMINISTRATIVE RESOURCES


Sakthivel Kailasam, Ying Wang, Jing-Chi Lo, Hsin-Fang Chang and Kuo-Chen Yeh* (2018) S-nitrosoglutathione works downstream of nitric oxide to mediate iron deficiency signaling in Arabidopsis The Plant Journal, DOI: 10.1111/tpj.13850

Cindy Ast, Jessica Foret, Luke M. Oltrogge, Roberto De Michele, Thomas J. Kleist, Cheng-Hsun Ho & Wolf B. Frommer (2017) Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins.Nature Communications(2017), doi:10.1038/s41467-017-00400-2

Chin-Hsien Tsai, Sheue-Fen Tzeng, Shih-Chuan Hsieh, Yu-Chih Yang, Yi-Wen Hsiao, Mong-Hsun Tsai, and Pei-Wen Hsiao (2017) A standardized herbal extract mitigates tumor inflammation and augments chemotherapy effect of docetaxel in prostate cancer. Scientific Reports 2017 Nov; 7: 15624

Jeng-Yuan Shiau, Kyoko Nakagawa-Goto, Kuo-Hsiung Lee, and Lie-Fen Shyur* (2017) Phytoagent deoxyelephantopin derivative inhibits triple negative breast cancer cell activity by inducing oxidative stress-mediated paraptosis-like cell death. Oncotarget, 2017, 8:56942-56958

Chin-Hsien Tsai, Sheue-Fen Tzeng, Shih-Chuan Hsieh, Chia-Jui Tsai, Yu-Chih Yang, Mong-Hsun Tsai and Pei-Wen Hsiao (2017) A Standardized Wedelia chinensis Extract Overcomes the Feedback Activation of HER2/3 Signaling upon Androgen-Ablation in Prostate Cancer. Frontiers in Pharmacology 2017 Oct; 8:721

Several studies elucidated the transcriptional responses to iron (Fe) starvation, however, the Fe sensing and signal transduction machineries are poorly resolved thus far in plants. With this focus, by using chemical biology approach, we identified a small molecule R7, whose use inhibited the physiological and molecular responses of Fe starvation in Arabidopsis. . ...more
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