網站分析程式 Academia Sinica Agricultural Biotechnology Research Center
 
 
FLASH logo
FLASH logo
中文 | HOME | ACADEMIA SINICA | SITEMAP  
ABRC information system  
line

 

NEWS more

line
HIGHLIGHTS
Crime scene reconstruction: determining time of RNA death by analysis of RNA degradation fragments
In addition to regulating RNA synthesis, increasing or decreasing RNA degradation is an alternative way to control gene expression. Moreover, RNA degradation plays an important role in plant defense against pathogens by directly attacking pathogen RNA or negatively regulating the expression of immune-related genes. Therefore, the study of RNA degradation mechanisms will increase understanding of crop defense mechanisms and lead to new approaches for gene expression control which will in turn provide new tools and research directions for the development of agricultural biotechnology. Scientists are currently able to use next generation sequencing technologies to globally detect RNA degradation fragments within cells. However, how to use RNA degradome data to determine the sequence features triggering RNA degradation, the proteins involved, as well as temporal understanding of RNA degradation occurrence remains obscure.

Dr. Ho-Ming Chen and her group at the Agricultural Biotechnology Research Center have developed novel approaches to analyze RNA degradome data. Their analyses have revealed that RNA-binding proteins can hinder RNA decay, leading to the accumulation of RNA degradation fragments at the binding sites of RNA-binding proteins which can be viewed as in vivo footprints of RNA-binding proteins. The properties of these RNA-binding proteins can be used to infer the state of RNA when degradation occurs.

In the recent Plant Cell paper published by Dr. Ho-Ming Chen’s group, they further demonstrate that the exon-junction complex (EJC) is also one of the RNA-binding proteins with the ability to stop RNA decay. Notably, EJCs are deposited onto RNA during RNA synthesis and maturation but are displaced by ribosomes in the pioneer round of translation. Therefore, EJC footprints in the RNA degradome can serve as a marker for RNA degradation before steady-state translation. EJC footprints were observed in the RNA degradomes of Arabidopsis, rice, worm and human cells. This finding will lead to novel approaches for identifying the RNAs that are rapidly degraded after synthesis and exploring the underlying mechanisms through RNA degradome analysis.

This paper was published and highlighted in the April issue of The Plant Cell in 2020. The first authors include Wen-Chi Lee, Bo-Han Hou, and Cheng-Yu Hou. This study was funded by the Agricultural Biotechnology Research Center, Academia Sinica.

Article link: http://www.plantcell.org/content/32/4/904

PHOTOS more

2020/11/11 ABRC Seminar

2020/11/09 ABRC Seminar 2020/10/19 ABRC Seminar 2020/08/24 ABRC Seminar

SEMINAR more

Young Scientist Forum more

李士畦副執行長 (工業技術研究院中分院 副執行長)
2021/03/08
11:00 AM
農業科技大樓1樓A134演講廳
農業廢剩物質循環模式與材料化應用

JOB OPENING more

ADMINISTRATIVE RESOURCES


Ya-Ru Li and Ming-Jung Liu (2020) Lost in translation of genetic information? Back to the beginning to pinpoint translation initiators. Genome Research, 30, 1418-1433

Surjit Singh, Sakthivel Kailasam, Jing-Chi Lo and Kuo-Chen Yeh* (2020) Histone H3 lysine4 trimethylation-regulated GRF11 expression is essential for the iron deficiency response in Arabidopsis thaliana. New Phytologist, https://doi.org/10.1111/nph.17130

Hui-Yu Chen, Yen-Ning Chen, Hung-Yu Wang, Zong-Ta Liu, Wolf B. Frommer & Cheng-Hsun Ho* (2020) Feedback inhibition of AMT1 NH4+-transporters mediated by CIPK15 kinase. BMC Biology, 2020 18:196

Size control study of microalga reveals secret of aberrant cell division!

Chang, H.-F.; Yang, P.-T.; Lin, H.-W.; Yeh, K.-C*; Chen, M.-N.; Wang, S.-L* (2020) Indium uptake and accumulation by rice and wheat and health risk associated with their consumption. Environmental Science & Technology, doi.org/10.1021/acs.est.0c02676

A mRNA is a stretch of letters (i.e. A/U/C/G nucleotides), which contains genetic information to build proteins. Similar to the 'text translation' (i.e. translating texts from one language to another), the process of using mRNA sequence information to produce proteins is called 'mRNA translation' in biology. ...more
line
Recommend browser :IE 8.0, Firefox3.0 / 1024x768
No. 128, Sec. 2, Academia Road, Nankang, Taipei, Taiwan 115
TEL: +886-2-2651-5910 FAX: +886-2-2651-5600
Copyright 2010 ABRC, Academia Sinica. All Rights Reserved