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Yu-Yi Wu, Bo-Han Hou, Wen-Chi Lee, Shin-Hua Lu, Chen-Jui Yang, Hervé Vaucheret and Ho-Ming Chen (2017) DCL2- and RDR6-dependent transitive silencing of SMXL4 and SMXL5 in Arabidopsis dcl4 mutants causes defective phloem transport and carbohydrate over-accumulation. The Plant Journal, 2017, 90(6):1064-1078
Dicer-Like (DCL) enzymes are able to process double-stranded RNA into small RNAs. Arabidopsis DCL4 and DCL2 each allow the post-transcriptional gene silencing (PTGS) of viruses and transgenes, but DCL2 activity is mostly obscured by DCL4. This hierarchy likely prevents DCL2 having any detrimental effects on endogenous genes. Indeed, dcl4 mutants exhibit leaf pigmentation under regular growth conditions. Here we report that the purple phenotype of dcl4 leaves correlates with carbohydrate over-accumulation and defective phloem transport, and depends on the activity of DCL2 and two enzymes, Suppressor of Gene Silencing 3 (SGS3) and RNA-Dependent RNA Polymerase 6 (RDR6), involved in double-stranded RNA synthesis. Further, this phenotype correlates with the down-regulation of two genes expressed in the apex and the vasculature, SMAX1-Like 4 (SMXL4) and SMXL5, and the accumulation of DCL2- and RDR6-dependent small interfering RNAs derived from these two genes. Supporting a causal effect, smxl4 smxl5 double mutants exhibit leaf pigmentation, enhanced starch accumulation and defective phloem transport, similar to dcl4 plants. Overall, this study elucidates the detrimental action of DCL2 when DCL4 is absent, and indicates that DCL4 outcompeting DCL2 in wild-type plants is crucial to prevent the degradation of endogenous transcripts by DCL2- and RDR6-dependent transitive PTGS. link

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*2017/11/23 10:30 AM
Dr. Wai-Leng Lee (Lecturer, School of Science, Monash University Malaysia)
Studies on treatment responsiveness of oral cancer cells
Auditorium A134, Agricultural Technology Building, Agricultural Biotechnology Research Center

*2017/11/27 10:30 AM
Dr. Kin-Ying To(Associate Specialist, Agricultural Biotechnology Research Center, Academia Sinica)
Research and Development of the ABRC Transformation Core Mainly for Non-model Plants
Auditorium A134, Agricultural Technology Building, Agricultural Biotechnology Research Center

*2017/12/18 10:30 AM
Dr. Anudep Rungsipipat (Associate Professor, Companion Animal Cancer Research Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand)
Chemopreventive Effects of Thai Herbs on Mice Induced Colorectal Carcinogenesis
Auditorium A134, Agricultural Technology Building, Agricultural Biotechnology Research Center

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Cindy Ast, Jessica Foret, Luke M. Oltrogge, Roberto De Michele, Thomas J. Kleist, Cheng-Hsun Ho & Wolf B. Frommer(2017) Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins.Nature Communications(2017), doi:10.1038/s41467-017-00400-2

Ji-Rong Yang, Chieh-Yu Cheng, Chih-Yuan Chen, Chao-Hua Lin, Chuan-Yi Kuo, Hsiang-Yi Huang, Fu-Ting Wu, Yu-Chih Yang, Chia-Ying Wu, Ming-Tsan Liu*, and Pei-Wen Hsiao* (2017) A virus-like particle vaccination strategy expands its tolerance to H3N2 antigenic drift by enhancing neutralizing antibodies against hemagglutinin stalk. Antiviral Research, 2017, 140: 62-75

Cheng-Hsun Ho* and Wolf B. Frommer (2016) Design and Functional Analysis of Fluorescent Nitrate and Peptide Transporter Acativity Sensors in Yeast Cultures Bio-protocol, vol 6, iss 3

Jeng-Yuan Shiau, Kyoko Nakagawa-Goto, Kuo-Hsiung Lee, and Lie-Fen Shyur* (2017) Phytoagent deoxyelephantopin derivative inhibits triple negative breast cancer cell activity by inducing oxidative stress-mediated paraptosis-like cell death. Oncotarget, 2017, 8:56942-56958

Yang S-Y, Huang T-K, Kuo H-F, Chiou T-J (2017) Role of vacuoles in phosphorus storage and remobilization Journal of Experimental Botany: erw481

Sensitivity, dynamic and detection range as well as exclusion of expression and instrumental artifacts are critical for the quantitation of data obtained with fluorescent protein (FP)-based biosensors in vivo. Current biosensors designs are, in general, unable to simultaneously meet all these criteria. Here, we describe a generalizable platform to create dual-FP biosensors with large dynamic ranges by employing a single FP-cassette, named GO-(Green-Orange) Matryoshka. The cassette nests a stable reference FP (large Stokes shift LSSmOrange) within a reporter FP (circularly permuted green FP). ...more
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