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Yen-Ling Lin, Chin-Lin Chung, Ming-Hui Chen, Chun-Han Chen, Su-Chiung Fang* (2020) SUMO protease SMT7 modulates ribosomal protein L30 to regulate cell-size checkpoint function. Plant Cell. 32(4): 1285-1307.
Proliferating cells actively coordinate growth and cell division to ensure cell-size homeostasis; however, the underlying mechanism through which size is controlled is poorly understood. Defect in a SUMO protease protein, suppressor of mat3 7 (SMT7), has been shown to reduce cell division number and increase cell size of the small-size mutant mating type locus 3-4 (mat3-4), which contains a defective retinoblastoma tumor suppressor-related protein of Chlamydomonas (Chlamydomonas reinhardtii). Here we describe development of an in vitro SUMOylation system using Chlamydomonas components and use it to provide evidence that SMT7 is a bona fide SUMO protease. We further demonstrate that the SUMO protease activity is required for supernumerous mitotic divisions of the mat3-4 cells. In addition, we identified RIBOSOMAL PROTEIN L30 (RPL30) as a prime SMT7 target and demonstrated that its SUMOylation is an important modulator of cell division in mat3-4 cells. Loss of SMT7 caused elevated SUMOylated RPL30 levels. Importantly, overexpression of the translational fusion version of RPL30-SUMO4, which mimics elevation of the SUMOylated RPL30 protein in mat3-4, caused a decrease in mitotic division and recapitulated the size-increasing phenotype of the smt7-1 mat3-4 cells. In summary, our study reveals a novel mechanism through which a SUMO protease regulates cell division in the mat3-4 mutant of Chlamydomonas and provides yet another important example of the role that protein SUMOylation can play in regulating key cellular processes, including cell division.

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2020/11/11 ABRC Seminar

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*2020/12/07 11:00 AM
Dr. Ming-Jung Liu (Assistant Research Fellow, Agricultural Biotechnology Research Center, Academia Sinica)
Lost in gene expression? Decipher the transcriptional and translational regulation for plant gene expressions
Auditorium A134, Agricultural Technology Building

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黃啟彰博士 (國立體育大學運動科學研究所 特聘教授兼研發長)
2020/12/28
11:00 AM
農業科技大樓1樓A134演講廳
運動營養增補劑之研發與商轉應用

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Ya-Ru Li and Ming-Jung Liu (2020) Lost in translation of genetic information? Back to the beginning to pinpoint translation initiators. Genome Research, 30, 1418-1433

Chang, H.-F.; Yang, P.-T.; Lin, H.-W.; Yeh, K.-C*; Chen, M.-N.; Wang, S.-L* (2020) Indium uptake and accumulation by rice and wheat and health risk associated with their consumption. Environmental Science & Technology, doi.org/10.1021/acs.est.0c02676

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Crime scene reconstruction: determining time of RNA death by analysis of RNA degradation fragments

Wen-Chi Lee, Bo-Han Hou, Cheng-Yu Hou, Shu-Ming Tsao, Ping Kao, Ho-Ming Chen* (2020) Widespread Exon Junction Complex Footprints in the RNA Degradome Mark mRNA Degradation before Steady State Translation. The Plant Cell, Vol. 32: 904–922.

A mRNA is a stretch of letters (i.e. A/U/C/G nucleotides), which contains genetic information to build proteins. Similar to the 'text translation' (i.e. translating texts from one language to another), the process of using mRNA sequence information to produce proteins is called 'mRNA translation' in biology. ...more
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